Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 91:451-454 (1989)
© 1989 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

DNA Methylation is Reduced in DNasel-Sensitive Regions of Plant Chromatin 1

Manfred Klaas2 and Richard M. Amasino3

Department of Biochemistry, College of Agricultural and Life Science, University of Wisconsin-Madison, Madison, Wisconsin 53706

Pea, barley, and corn chromatin was isolated and subjected to limited digestion with DNasel. The preferentially degraded, small molecular weight fraction, presumably consisting of sequences in an active state of expression, was isolated and its 5-methylcytosine content determined. The DNasel-sensitive chromatin fraction from all three plant species investigated contained a markedly reduced level of DNA methylation compared to total DNA.

2 Supported by a postdoctoral fellowship grant from the "Sonderprogramm Gentechnologie" of the Deutscher Akademischer Austauschdienst (DAAD).

3 Scholar of the James D. and Dorothy Shaw Scholar's Program.

1 This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison, grant DCB-8602500 from the National Science Foundation and a Steenbock Career Development Award to R. M. A.




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A Castilho, N Neves, M Rufini-Castiglione, W Viegas, and J. Heslop-Harrison
5-Methylcytosine distribution and genome organization in triticale before and after treatment with 5-azacytidine
J. Cell Sci., January 12, 1999; 112(23): 4397 - 4404.
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