Plant Physiology 91:514-525 (1989)
© 1989 American Society of Plant Biologists
Metabolism and Enzymology
Isolation and Characterization of High CO2-Requiring-Mutants of the Cyanobacterium Synechococcus PCC7942 1
Two Phenotypes that Accumulate Inorganic Carbon but Are Apparently Unable to Generate CO2 within the Carboxysome
G. D. Price and
M. R. Badger
Plant Environmental Biology Group, Research School of Biological Sciences, Australian National University, P. O. Box 475, Canberra City, A.C.T. 2601, Australia
A total of 24 high CO2-requiring-mutants of the cyanobacterium Synechococcus PCC7942 have been isolated and partially characterized. These chemically induced mutants are able to grow at 1% CO2, on agar media, but are incapable of growth at air levels of CO2. All the mutants were able to accumulate inorganic carbon (Ci) to levels similar to or higher than wild type cells, but were apparently unable to generate intracellular CO2. On the basis of the rate of Ci release following a light (5 minutes)  dark transition two extreme phenotypes (fast and slow release mutants) and a number of `intermediate' mutants (normal release) were identified. Compared to wild-type cells, Type I mutants had the following characteristics: fast Ci release, normal internal Ci pool, normal carbonic anhydrase (CA) activity in crude extracts, reduced internal exchange of 18O from 18O-labeled CO2, 1% CO2 requirement for growth in liquid media, normal affinity of carboxylase for CO2, and long, rod-like carboxysomes. Type II mutants had the following characteristics: slow Ci release, increased internal Ci pool, normal CA activity in crude extracts, normal internal 18O exchange, a 3% CO2 requirement for growth in liquid media, high carboxylase activity, normal affinity of carboxylase for CO2, and normal carboxysome structure but increased in numbers per cell. Both mutant phenotypes appear to have genetic lesions that result in an inability to convert intracellular HCO3– to CO2 inside the carboxysome. The features of the type I mutants are consistent with a scenario where carboxysomal CA has been mistargeted to the cytosol. The characteristics of the type II phenotype appear to be most consistent with a scenario where CA activity is totally missing from the cell except for the fact that cell extracts have normal CA activity. Alternatively the type II mutants may have a lesion in their capacity for H+ import during photosynthesis.
1 This work was supported by a National Research Followup (to G. D. P.) awarded by the Australian Government Department of Education, Employment and Training.
This article has been cited by other articles:
|
|
|
|
 
G. D. Price, M. R. Badger, F. J. Woodger, and B. M. Long
Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants
J. Exp. Bot.,
May 1, 2008;
59(7):
1441 - 1461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Dou, S. Heinhorst, E. B. Williams, C. D. Murin, J. M. Shively, and G. C. Cannon
CO2 Fixation Kinetics of Halothiobacillus neapolitanus Mutant Carboxysomes Lacking Carbonic Anhydrase Suggest the Shell Acts as a Diffusional Barrier for CO2
J. Biol. Chem.,
April 18, 2008;
283(16):
10377 - 10384.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Long, M. R. Badger, S. M. Whitney, and G. D. Price
Analysis of Carboxysomes from Synechococcus PCC7942 Reveals Multiple Rubisco Complexes with Carboxysomal Proteins CcmM and CcaA
J. Biol. Chem.,
October 5, 2007;
282(40):
29323 - 29335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. V. Moroney and R. A. Ynalvez
Proposed Carbon Dioxide Concentrating Mechanism in Chlamydomonas reinhardtii
Eukaryot. Cell,
August 1, 2007;
6(8):
1251 - 1259.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Emlyn-Jones, F. J. Woodger, G. D. Price, and S. M. Whitney
RbcX Can Function as a Rubisco Chaperonin, But is Non-Essential in Synechococcus PCC7942
Plant Cell Physiol.,
December 1, 2006;
47(12):
1630 - 1640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Woodger, M. R. Badger, and G. D. Price
Inorganic Carbon Limitation Induces Transcripts Encoding Components of the CO2-Concentrating Mechanism in Synechococcus sp. PCC7942 through a Redox-Independent Pathway
Plant Physiology,
December 1, 2003;
133(4):
2069 - 2080.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Kusian, D. Sultemeyer, and B. Bowien
Carbonic Anhydrase Is Essential for Growth of Ralstonia eutropha at Ambient CO2 Concentrations
J. Bacteriol.,
September 15, 2002;
184(18):
5018 - 5026.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Cannon, C. E. Bradburne, H. C. Aldrich, S. H. Baker, S. Heinhorst, and J. M. Shively
Microcompartments in Prokaryotes: Carboxysomes and Related Polyhedra
Appl. Envir. Microbiol.,
December 1, 2001;
67(12):
5351 - 5361.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Van, Y. Wang, Y. Nakamura, and M. H. Spalding
Insertional Mutants of Chlamydomonas reinhardtii That Require Elevated CO2 for Survival
Plant Physiology,
October 1, 2001;
127(2):
607 - 614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Bobik, G. D. Havemann, R. J. Busch, D. S. Williams, and H. C. Aldrich
The Propanediol Utilization (pdu) Operon of Salmonella enterica Serovar Typhimurium LT2 Includes Genes Necessary for Formation of Polyhedral Organelles Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation
J. Bacteriol.,
October 1, 1999;
181(19):
5967 - 5975.
[Abstract]
[Full Text]
|
|
|
|
|
