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Plant Physiology 91:581-586 (1989)
© 1989 American Society of Plant Biologists

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Environmental and Stress Physiology

Pyrroline-5-Carboxylate Reductase Is in Pea (Pisum sativum L.) Leaf Chloroplasts 1

P. John Rayapati, Cecil R. Stewart and Ethan Hack

Department of Botany, Iowa State University, Ames, Iowa 50011

Proline accumulation is a well-known response to water deficits in leaves. The primary cause of accumulation is proline synthesis. {Delta}1-Pyrroline-5-carboxylate reductase (PCR) catalyzes the final reaction of proline synthesis. To determine the subcellular location of PCR, protoplasts were made from leaves of Pisum sativum L., lysed, and fractionated by differential and Percoll density gradient centrifugation. PCR activity comigrated on the gradient with the activity of the chloroplast stromal marker NADPH-dependent triose phosphate dehydrogenase. We conclude that PCR is located in chloroplasts, and therefore that chloroplasts can synthesize proline. PCR activities from chloroplasts and etiolated shoots were compared. PCR activity from both extracts is stimulated at least twofold by 100 millimolar KCl or 10 millimolar MgCl2. The pH profiles of PCR activity from both extracts reveal two separate optima at pH 6.5 and 7.5. Native isoelectric focusing gels of sampies from etiolated tissue reveal a single band of PCR activity with a pl of 7.8.

1 Supported by U.S. Department of Agriculture Competitive Research Grants Office grant 85-CRCR-1-1671.




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 Plant Cell PhysiolHome page
M. Murahama, T. Yoshida, F. Hayashi, T. Ichino, Y. Sanada, and K. Wada
Purification and Characterization of {Delta}1-Pyrroline-5-Carboxylate Reductase Isoenzymes, Indicating Differential Distribution in Spinach (Spinacia oleracea L.) Leaves
Plant Cell Physiol., July 1, 2001; 42(7): 742 - 750.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Society of Plant Biologists