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Plant Physiology 91:1006-1013 (1989)
© 1989 American Society of Plant Biologists

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Cellular and Structural Biology

An Abundant, Highly Conserved Tonoplast Protein in Seeds 1

Kenneth D. Johnson2, Eliot M. Herman and Maarten J. Chrispeels

Department of Biology, University of California, San Diego, La Jolla, California 92093, Plant Molecular Biology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705

We have isolated the membranes of the protein storage vacuoles (protein bodies) from Phaseolus vulgaris cotyledons and purified an integral membrane protein with Mr 25,000 (TP 25). Antiserum to TP 25 recognizes an abundant polypeptide in the total cell extracts of many different seeds (monocots, dicots, and a gymnosperm), and specifically labels the vacuolar membranes of thin-sectioned soybean embryonic axes and cotyledons. TP 25 was not found in the starchy endosperm of barley and wheat or the seed coats of bean but was present in all seed parts examined that consist of living cells at seed maturity. The abundance of TP 25 was not correlated with the amount of storage protein in seed tissue, and the protein was not found in leaves that accumulate leaf storage protein. On the basis of its abundance, evolutionary conservation, and distribution in the plant, we propose that TP 25 may play a role in maintaining the integrity of the tonoplast during the dehydration/rehydration sequence of seeds.

2 On leave from the Department of Biology, San Diego State University, San Diego, CA 92182.

1 Supported by grants to M. J. C. from the National Science Foundation (Metabolic Biology) and to E. M. H. and M. J. C. from the U.S. Department of Agriculture (Genetic mechanisms for crop improvement).




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Copyright © 1989 by the American Society of Plant Biologists