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Metabolism and Enzymology

Blue-Light Regulation of Specific Transcript Levels in Pisum sativum¹

Fluence-Response, Time-Course, and Reciprocity Characteristics

Department of Biological Sciences, University of Illinois at Chicago, Box 4348, m/c 067, Chicago, Illinois 60680, Laboratory for Cell, Molecular, and Developmental Biology, University of Illinois at Chicago, Box 4348, m/c 067, Chicago, Illinois 60680

The expression of many nuclear genes in plants is light regulated. We have examined the fluence-response, time-course, and reciprocity characteristics of four nuclear, blue-light-regulated transcripts in Pisum sativum L. var Alaska: Cab RNA, pEA207 RNA, pEA215 RNA, and pEA25 RNA. To avoid complications due to blue-light-induced transformation of phytochrome, we have adapted the procedure of measuring blue-light-induced changes in steady-state-RNA levels in seedlings grown in continuous red light. The fluence-response curves for Cab and pEA215 RNA are bell-shaped, with peak accumulations at 10² and 10¹ micromoles per square meter, respectively. No response is observed at 10⁴ micromoles per square meter. pEA25 RNA has threshold and saturation fluences below 10^–1 micromoles per square meter. pEA207 RNA has a threshold at 10² micromoles per square meter and saturates above 10⁴ micromoles per square meter. pEA215 and Cab RNA start to increase within 1 hour after the 10¹ micromoles per square meter pulse, and finish accumulation by 5 hours. The decrease in pEA207 RNA in response to 10⁴ micromoles per square meter and pEA25 RNA in response to 10¹ or 10⁴ micromoles per square meter starts between 3 and 5 hours after the blue-light pulse. The Bunsen-Roscoe Law of Reciprocity is followed in the Cab, pEE215, pEA25, and pEA207 RNA responses to 10¹ and 10⁴ micromoles per square meter.

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