Plant Physiol.
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Plant Physiology 91:1056-1062 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Subunit Composition of Glutamine Synthetase Isozymes from Root Nodules of Bean (Phaseolus vulgaris L.) 1

Xiaoyin Cai and Peter P. Wong

Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas 66506

Glutamine synthetase from bean nodules can be separated into two isoforms, GSn1 and GSn2. A purification protocol has been developed. It included protamine sulfate precipitation, ammonium sulfate fractionation, anthranilate-affinity chromatography, Dye-Matrex (Orange A) chromatography, and diethylaminoethyl-cellulose ion-exchange chromatography. GSn1 and GSn2 have been purified to homogeneity. Subunit structure analysis using two-dimensional polyacrylamide gel electrophoresis revealed that GSn1 was composed of two different types of subunit polypeptides. They differed in isoelectric points (6.0 and 6.3) but had the same molecular weights (46,000 Daltons). GSn2 was composed of only one type of subunit polypeptide. It had an isoelectric point of 6.0 and a molecular weight of 46,000 Daltons. It was apparently identical to one of the polypeptides found in GSn1. Glutamine synthetase holoenzyme consisted of eight subunits. In the nodule there are two different types of glutamine synthetase subunit polypeptides. Random combinations of the polypeptides should generate nine different isozymes. Our electrophoretic analysis revealed that GSn2 was but one of the isozymes, and GSn1 was a composite of the other eight. Hence, nodule glutamine synthetase isozymes were homo-octameric as well as hetero-octameric.

1 Supported by U.S. Department of Agriculture, Competitive Research Grants Program Grant 86-CRCR-1-2116, 88-37120-3891, and NASA Contract NAGW-1197. Contribution No. 89-532-J from the Kansas Agricultural Experiment Station.






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Copyright © 1989 by the American Society of Plant Biologists