Plant Physiology 91:947-953 (1989)
© 1989 American Society of Plant Biologists
Metabolism and Enzymology
Nitrate Transport Is Independent of NADH and NAD(P)H Nitrate Reductases in Barley Seedlings 1
Robert L. Warner and
Ray C. Huffaker
Department of Agronomy and Soils, Washington State University, Pullman, Washington 99164-6420,
Plant Growth Laboratory and the Department of Agronomy & Range Science, University of California, Davis, California 95616
Barley (Hordeum vulgare L.) has NADH-specific and NAD(P)H-bispecific nitrate reductase isozymes. Four isogenic lines with different nitrate reductase isozyme combinations were used to determine the role of NADH and NAD(P)H nitrate reductases on nitrate transport and assimilation in barley seedlings. Both nitrate reductase isozymes were induced by nitrate and were required for maximum nitrate assimilation in barley seedlings. Genotypes lacking the NADH isozyme (Az12) or the NAD(P)H isozyme (Az70) assimilated 65 or 85%, respectively, as much nitrate as the wild type. Nitrate assimilation by genotype (Az12;Az70) which is deficient in both nitrate reductases, was only 13% of the wild type indicating that the NADH and NAD(P)H nitrate reductase isozymes are responsible for most of the nitrate reduction in barley seedlings. For all genotypes, nitrate assimilation rates in the dark were about 55% of the rates in light. Hypotheses that nitrate reductase has direct or indirect roles in nitrate uptake were not supported by this study. Induction of nitrate transporters and the kinetics of net nitrate uptake were the same for all four genotypes indicating that neither nitrate reductase isozyme has a direct role in nitrate uptake in barley seedlings.
1 Scientific Paper No. 8901-02. College of Agriculture and Home Economics Research Center, Washington State University, Research Project No. 0233. This work was supported in part by National Science Foundation grant DMB 85-05095.
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