Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 91:1323-1328 (1989)
© 1989 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lewinsohn, E.
Right arrow Articles by Gressel, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewinsohn, E.
Right arrow Articles by Gressel, J.
Agricola
Right arrow Articles by Lewinsohn, E.
Right arrow Articles by Gressel, J.
Metabolism and Enzymology

Flavanone Glycoside Biosynthesis in Citrus

Chalcone Synthase, UDP-Glucose:Flavanone-7-O-Glucosyl-Transferase and -Rhamnosyl-Transferase Activities in Cell-Free Extracts

Efraim Lewinsohn1, Lothar Britsch, Yehuda Mazur2 and Jonathan Gressel3

Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, IL-76100, Israel, Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, IL-76100, Israel, Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II, der Albert-Ludwigs Universität, D-7800 Freiburg i. Br., Federal Republic of Germany

Previous indirect evidence suggested that the biosynthesis of flavonoids in Citrus may not proceed via the usual chalcone synthase reaction and that glycosylation occurs during chalcone formation and not afterward, as has been reported in other species. We detected chalcone-synthase and UDP-glucose:flavanone-7-O-glucosyl-transferase activities in cell-free extracts of Citrus. The glucosylated flavanone was further rhamnosylated when exogenous UDP-glucose and NADPH were added to the extract. Chalcone-synthase activity was detected in cell-free extracts derived from young leaves and fruits. Young fruits (2 millimeter diameter) had the highest chalcone synthase activity. UDP-glucose:flavanone-7-O-glucosyl-transferase activity was measured in cell-free extracts derived from young leaves and fruits of Citrus mitis and Citrus maxima. The highest UDP-glucose:flavanone-7-O-glucosyl-transferase activity was found in young C. maxima leaves. These data indicate that Citrus contains a flavonoid pathway similar to that studied in other species.

1 Supported by a Minerva foundation travel grant. Present address: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340.

2 Rebecca and Israel Sieff Chair of Organic Chemistry.

3 Gilbert de Botton Chair of Plant Science.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. M. Ford, P. K. Boss, and P. B. Hoj
Cloning and Characterization of Vitis vinifera UDP-Glucose:Flavonoid 3-O-Glucosyltransferase, a Homologue of the Enzyme Encoded by the Maize Bronze-1 Locus That May Primarily Serve to Glucosylate Anthocyanidins in Vivo
J. Biol. Chem., April 10, 1998; 273(15): 9224 - 9233.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1989 by the American Society of Plant Biologists