Plant Physiology 92:123-129 (1990)
© 1990 American Society of Plant Biologists
Membranes and Bioenergetics
Amino Acid Transport across the Tonoplast of Vacuoles Isolated from Barley Mesophyll Protoplasts 1
Uptake of Alanine, Leucine, and Glutamine
Karl-Josef Dietz,
Renate Jäger,
Georg Kaiser2 and
Enrico Martinoia
Institut für Botanik and Pharmazeutische Biologie der Universität, Mittlerer Dallenbergweg 64, 8700 Würzburg, Federal Republic of Germany,
Institut für Pflanzenwissenschaften, ETH Zürich, CH-8092 Zürich, Switzerland
Mesophyll protoplasts from leaves of well-fertilized barley (Hordeum vulgare L.) plants contained amino acids at concentrations as high as 120 millimoles per liter. With the exception of glutamic acid, which is predominantly localized in the cytoplasm, a major part of all other amino acids was contained inside the large central vacuole. Alanine, leucine, and glutamine are the dominant vacuolar amino acids in barley. Their transport into isolated vacuoles was studied using 14C-labeled amino acids. Uptake was slow in the absence of ATP. A three- to sixfold stimulation of uptake was observed after addition of ATP or adenylyl imidodiphosphate an ATP analogue not being hydrolyzed by ATPases. Other nucleotides were ineffective in increasing the rate of uptake. ATP-Stimulated amino acid transport was not dependent on the transtonoplast pH or membrane potential. p-Chloromercuriphenylsulfonic acid and n-ethyl maleimide increased transport independently of ATP. Neutral amino acids such as valine or leucine effectively decreased the rate of alanine transport. Glutamine and glycine were less effective or not effective as competitive inhibitors of alanine transport. The results indicate the existence of a uniport translocator specific for neutral or basic amino acids that is under control of metabolic effectors.
2 Present address: Stiftung Volkswagenwerk, Kastanienallee 35, 3000 Hannover 35, F.R.G.
1 This work was supported by the Deutsche Forschungsgemeinschaft within the Sonderforschungsbereich 176 of the Bayerische Julius-Maximilians-Universität Würzburg.
This article has been cited by other articles:
|
|
|
|
 
S. S. Sharma and K.-J. Dietz
The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress
J. Exp. Bot.,
March 1, 2006;
57(4):
711 - 726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Esposito, G. Guerriero, V. Vona, V. Di Martino Rigano, S. Carfagna, and C. Rigano
Glutamate synthase activities and protein changes in relation to nitrogen nutrition in barley: the dependence on different plastidic glucose-6P dehydrogenase isoforms
J. Exp. Bot.,
January 1, 2005;
56(409):
55 - 64.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Su, W. B. Frommer, and U. Ludewig
Molecular and Functional Characterization of a Family of Amino Acid Transporters from Arabidopsis
Plant Physiology,
October 1, 2004;
136(2):
3104 - 3113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Martinoia, A. Massonneau, and N. Frangne
Transport Processes of Solutes across the Vacuolar Membrane of Higher Plants
Plant Cell Physiol.,
November 1, 2000;
41(11):
1175 - 1186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Aubert, G. Curien, R. Bligny, E. Gout, and R. Douce
Transport, Compartmentation, and Metabolism of Homoserine in Higher Plant Cells . Carbon-13- and Phosphorus-31-Nuclear Magnetic Resonance Studies
Plant Physiology,
February 1, 1998;
116(2):
547 - 557.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Roos, R. Schulze, and J. Steighardt
Dynamic Compartmentation of Vacuolar Amino Acids in Penicillium cyclopium. CYTOSOLIC ADENYLATES ACT AS A CONTROL SIGNAL FOR EFFLUX INTO THE CYTOSOL
J. Biol. Chem.,
June 20, 1997;
272(25):
15849 - 15855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
