This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (55) Citing Articles via Google Scholar Google Scholar Articles by Bedinger, P. A. Articles by Edgerton, M. D. Search for Related Content PubMed PubMed Citation Articles by Bedinger, P. A. Articles by Edgerton, M. D. Agricola Articles by Bedinger, P. A. Articles by Edgerton, M. D.

Development and Growth Regulation

Developmental Staging of Maize Microspores Reveals a Transition in Developing Microspore Proteins ¹

Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280

A method for the preparation of developmentally staged microspores and young pollen from maize (Zea mays) has been devised. The preparations are of sufficient purity and quantity for biochemical analysis, including the analysis of steady-state protein and RNA populations associated with each stage. A major transition in protein populations occurs during the developmental period that encompasses microspore mitosis, the asymmetric nuclear division producing the vegetative and generative nuclei. Several differences between early and late stage proteins can be detected by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two-dimensional gel electrophoresis of proteins reveals that over half of the steady-state proteins differ between the younger and older stages, either quantitative or qualitative. One protein that increases in relative abundance about fourfold is actin. In vitro translation of RNA isolated from staged microspores demonstrates changes in microspore gene expression during the same developmental period.

This article has been cited by other articles:

				M. L. Engel, R. Holmes-Davis, and S. McCormick Green Sperm. Identification of Male Gamete Promoters in Arabidopsis Plant Physiology, August 1, 2005; 138(4): 2124 - 2133. [Abstract] [Full Text] [PDF]

				J. Zhao, W. Newcomb, and D. Simmonds Heat-Shock Proteins 70 kDa and 19 kDa are not Required for Induction of Embryogenesis of Brassica napus L. cv. Topas Microspores Plant Cell Physiol., December 15, 2003; 44(12): 1417 - 1421. [Abstract] [Full Text] [PDF]

				R. Datta, K. C. Chamusco, and P. S. Chourey Starch Biosynthesis during Pollen Maturation Is Associated with Altered Patterns of Gene Expression in Maize Plant Physiology, December 1, 2002; 130(4): 1645 - 1656. [Abstract] [Full Text] [PDF]