Plant Physiology 92:637-641 (1990)
© 1990 American Society of Plant Biologists
Metabolism and Enzymology
Subcellular Compartmentation of Uridine Nucleotides and Nucleoside-5' -Diphosphate Kinase in Leaves 1
Jane Dancer,
H. Ekkehard Neuhaus and
Mark Stitt
Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, 8580 Bayreuth, Federal Republic of Germany
The subcellular compartmentation of nucleoside diphosphate kinase (EC 2.7.4.6) and the uridine nucleotides has been studied in leaves. Membrane filtration of barley (Hordeum vulgare L.) leaf mesophyll protoplasts and differential centrifugation of spinach (Spinacia oleracea L.) leaf extracts showed that about half the nucleoside diphosphate kinase is present in the cytosol. The activity is adequate to account for the turnover of UTP and UDP during photosynthetic sucrose synthesis. Nonaqueous density gradient centrifugation of freeze-stopped, lyophilized spinach leaf material showed that the uridine nucleotides are predominantly located in the cytosol and that the cytosolic UDP-glucose pool is considerably larger than the UTP or UDP pools.
1 This research was supported by the Deutsche Forschungsgemeinschaft (SFB 137).
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