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Metabolism and Enzymology

Purification and Partial Characterization of a Fructanase which Hydrolyzes Natural Polysaccharides from Sugarcane Juice ¹

Laboratory of Plant Physiology, Faculty of Biology, Complutense University, 28040 Madrid, Spain, Department of Plant Physiology, Faculty of Biology, La Havanne University, Cuba

A new sugarcane (Saccharum officinarum L.) fructanase which hydrolyzes both high molecular weight polysaccharides |Fructose₄:Galactitol₅|_n (SP) and moderate-sized carbohydrates |Fructose₂:Galactitol₃3|_n (MMWC) has been purified from sugarcane juice. The K_m value has been estimated to be 33.7 micrograms per milliliter and 20 micrograms per milliliter for SP and MMWC, respectively. The optimal pH and temperature values are 6.0 and 30°C, respectively. Purified protein has a pl value of 6.35 and a molecular weight of 13.2 kilodaltons. Fructanase activity appears to be Mn²⁺-dependent.