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Metabolism and Enzymology

Oxygen and Hydrogen Isotope Fractionation during Cellulose Metabolism in Lemna gibba L. ¹

Department of Earth and Space Science, University of California Los Angeles, Los Angeles, California 90024

Lemna gibba L. B3 was grown under heterotrophic, photoheterotrophic, and autotrophic conditions in water having a variety of hydrogen and oxygen isotopic compositions. The slopes of the linear regression lines between the isotopic composition of water and leaf cellulose indicated that under the three growth conditions about 40, 70, and 100% of oxygens and carbon-bound hydrogens of cellulose exchanged with those of water prior to cellulose formation. Using the equations of the linear relationships, we estimated the overall fractionation factors between water and the exchanged oxygen and carbon bound-hydrogen of cellulose. At least two very different isotope effects must determine the hydrogen isotopic composition of Lemna cellulose. One reflects the photosynthetic reduction of NADP, while the second reflects exchange reactions that occur subsequent to NADP reduction. Oxygen isotopic composition of cellulose apparently is determined by a single type of exchange reaction with water. Under different growth conditions, variations in metabolic fluxes affect the hydrogen isotopic composition of cellulose by influencing the extent to which the two isotope effects mentioned above are recorded. The oxygen isotopic composition of cellulose is not affected by such changes in growth conditions.

³ Present address: Department of Earth Sciences, University of California, Santa Barbara, CA 93106.

¹ Supported by National Science Foundation grants DMB 84-05003 and DMB 88-96201, U.S. Department of Energy grant DE-87ER60615, and United States-Israel Binational Agricultural Research and Development Fund grant SI-0024-85.

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