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Plant Physiology 93:353-355 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Fructokinases from Developing Maize Kernels Differ in Their Specificity for Nucleoside Triphosphates

Douglas C. Doehlert

Seed Biosynthesis Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, 1815 North University Street, Peoria, Illinois 61604

A new form of fructokinase has been identified from developing maize (Zea mays L.) kernels that utilizes CTP, UTP, and GTP from four to eight times more effectively than ATP at nonlimiting concentrations. Ten millimolar dithiothreitol was necessary to stabilize activity. A second form of fructokinase was nonspecific for nucleoside triphosphate whereas a third form was fairly specific for ATP.





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S. Odanaka, A. B. Bennett, and Y. Kanayama
Distinct Physiological Roles of Fructokinase Isozymes Revealed by Gene-Specific Suppression of Frk1 and Frk2 Expression in Tomato
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W. S. da-Silva, G. L. Rezende, and A. Galina
Subcellular distribution and kinetic properties of cytosolic and non-cytosolic hexokinases in maize seedling roots: implications for hexose phosphorylation
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Copyright © 1990 by the American Society of Plant Biologists