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Plant Physiology 93:67-71 (1990)
© 1990 American Society of Plant Biologists

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Development and Growth Regulation

Cytokinins in Vegetative and Reproductive Buds of Pseudotsuga menziesii1

John W. Morris2, Patrick Doumas3, Roy O. Morris2 and Joe B. Zaerr

Department of Forest Science, Oregon State University, Corvallis, Oregon 97331

Immunoaffinity techniques using columns of immobilized antibodies raised against zeatin riboside and isopentenyladenosine were found to be effective in isolating cytoklnins from vegetative, female, and male buds of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco). The purified cytokinins were separated by reverse phase high performance liquid chromatography and analyzed by radioimmunoassay. Confirmation of cytokinin identities was by gas chromatography-mass spectrometry. Immediately prior to bud burst, all bud types contained three major cytokinins: isopentenyladenosine, zeatin riboside, and a hexose conjugate of zeatin riboside (not zeatin riboside O-glucoside). Zeatin-type cytokinins were present in relatively high concentration in vegetative and female buds. In male buds, however, relatively high levels of isopentenyladenosine were found together with low levels of zeatin-type cytokinins.

2 Present address: Department of Biochemistry, University of Missouri, Columbia, MO 65211.

3 Present address: Institut National de la Recherche Agronomique, Station d'Amelioration des Arbres Forestiers, Ardon 45160 Olivet, France.

1 Paper 2544 of the Forest Research Laboratory, Oregon State University, Corvallis, OR 97331.




This article has been cited by other articles:


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L. Kong, S. R. Abrams, S. J. Owen, A. Van Niejenhuis, and P. Von Aderkas
Dynamic changes in concentrations of auxin, cytokinin, ABA and selected metabolites in multiple genotypes of Douglas-fir (Pseudotsuga menziesii) during a growing season
Tree Physiol, February 1, 2009; 29(2): 183 - 190.
[Abstract] [Full Text] [PDF]


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Copyright © 1990 by the American Society of Plant Biologists