Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 93:484-488 (1990)
© 1990 American Society of Plant Biologists

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Environmental and Stress Physiology

Effect of Glutathione on Phytochelatin Synthesis in Tomato Cells 1

Mary Lou Mendum2, Subhash C. Gupta3 and Peter B. Goldsbrough

Department of Horticulture, Purdue University, West Lafayette, Indiana 47907

Growth of cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, in the presence of cadmium is inhibited by buthionine sulfoximine, an inhibitor of glutathione synthesis. Cell growth and phytochelatin synthesis are restored to cells treated with buthionine sulfoximine by the addition of glutathione to the medium. Glutathione stimulates the accumulation of phytochelatins in cadmium treated cells, indicating that availability of glutathione can limit synthesis of these peptides. Exogenous glutathione causes a disproportionate increase in the level of smaller phytochelatins, notably [{gamma}-Glu-Cys]2-Gly. In the presence of buthionine sulfoximine and glutathione, phytochelatins that are produced upon exposure to cadmium incorporate little [35S]cysteine, indicating that these peptides are probably not synthesized by sequential addition of cysteine and glutamate to glutathione.

2 Present address: Department of Viticulture and Enology, University of California, Davis, CA 95616.

3 Present address: ARS, Northern Region Research Center, 1815 N. University St., Peoria, IL 61604.

1 Supported in part by U.S. Department of Agriculture grant No. 85-CRCR-1-1653. Journal paper No. 12,318 of the Purdue University Agricultural Experiment Station.




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M. Inouhe, R. Ito, S. Ito, N. Sasada, H. Tohoyama, and M. Joho
Azuki Bean Cells Are Hypersensitive to Cadmium and Do Not Synthesize Phytochelatins
Plant Physiology, July 1, 2000; 123(3): 1029 - 1036.
[Abstract] [Full Text]


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