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Environmental and Stress Physiology

A Direct Screening Procedure for Gravitropism Mutants in Arabidopsis thaliana (L.) Heynh. ¹

Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

In order to isolate gravitropism mutants of Arabidopsis thaliana (L.) Heynh. var Estland for the genetic dissection of the gravitropism pathway, a direct screening procedure has been developed in which mutants are selected on the basis of their gravitropic response. Variability in hypocotyl curvature was dependent on the germination time of each seed stock, resulting in the incorrect identification of several lines as gravitropism mutants when a standard protocol for the potentiation of germination was used. When the protocol was adjusted to allow for differences in germination time, these lines were eliminated from the collection. Out of the 60,000 M2 seedlings screened, 0.3 to 0.4% exhibited altered gravitropism. In approximately 40% of these mutant lines, only gravitropism by the root or the hypocotyl was altered, while the response of the other organ was unaffected. These data support the hypothesis that root and hypocotyl gravitropism are genetically separable.

¹ Supported by the U.S. National Aeronautics and Space Administration under grants NAGW-882 to K. P., NGT-50241 to B. B. and NAGW-70 to S. B., and by the U.S. Department of Energy under contract DE-ACO2-76ERO-1338.

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