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Molecular Biology and Gene Regulation

Characterization of the Expression of the Petunia Glycine-Rich Protein-1 Gene Product ¹

Department of Plant Science, University of Nevada-Reno, Reno, Nevada 89557, Department of Biochemistry, University of Nevada-Reno, Reno, Nevada 89557, Department of Genetics, University of Georgia, Athens, Georgia 30602

We have examined the expression of the petunia (Petunia hybrida) glycine-rich protein-1 (ptGRP1) gene product using an antibody raised against a synthetic peptide comprising amino acids 22 through 36 of the mature ptGRP1 protein. This antibody recognizes a single protein of 23 kilodaltons. Cell fractionation studies showed that, as predicted (CM Condit, RB Meagher [1986] Nature 323: 178-181), ptGRP1 is most likely localized in the cell wall. In addition, it was found that (extractable) ptGRP1 is present in much higher abundance in unexpanded than in fully expanded tissue, with highest levels of accumulation in the bud. This same developmentally regulated pattern of protein expression was found in all varieties of petunia tested. In addition, tissue blots of petunia stem sections showed that ptGRP1 is localized to within the vascular tissue (to at least the phloem or cambium) and to either the epidermal cells or to a layer of collenchyma cells directly below the epidermis. Localization of ptGRP1 antigen in these cell types is shown to occur at different times in the overall development of the plant and at different quantitative levels.

¹ This work was funded by National Science Foundation grant DCB-8615798. A contribution of the Nevada Agricultural Experimental Station.

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