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Molecular Biology and Gene Regulation

Photoregulation of -Tubulin mRNA Abundance in Etiolated Oat and Barley Seedlings ¹

Department of Biology, Colorado State University, Ft. Collins, Colorado 80523

The effect of light on the abundance of -tubulin mRNA was measured in etiolated Avena sativa L. and Hordeum vulgare L. seedlings. Slot blot analysis employing an oat -tubulin cDNA clone was used to measure -tubulin mRNA levels. White light induced a 45% decrease in oat -tubulin mRNA abundance by 2 hours after transfer. A saturating red light pulse induced 40 and 55% decreases in -tubulin mRNA levels in oats and barley, respectively. Recovery of -tubulin mRNA levels was observed after a red light pulse but not after transfer to continuous white light. The red light induced decrease in oat -tubulin mRNA abundance was not reversible by a subsequent far-red light treatment. The mesocotyl portion of etiolated oat seedlings exhibited a more dramatic decrease in -tubulin mRNA abundance in response to red light than did the coleoptile portion. The results indicate that the well-documented effects of red light on the growth of etiolated seedlings are accompanied by changes in the expression of the -tubulin genes.

³ Present address: Department of Agronomy, Colorado State University, Ft. Collins, CO 80523.

⁴ Present address: Chinese Academy of Agricultural Sciences, Beijing, China.

¹ Research supported by the Colorado State University Agricultural Experiment Station and by U.S. Department of Agriculture Competitive Research Grants Office Grant No. 88-37261-3681.

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