Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 94:132-142 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Biosynthetic Origin of Gibberellins A3 and A7 in Cell-Free Preparations from Seeds of Marah macrocarpus and Malus domestica1

Kumyul S. Albone, Paul Gaskin, Jake MacMillan, Bernard O. Phinney and Christine L. Willis

School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom, Department of Biology, U.C.L.A., Los Angeles, California 90024

Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A9 (GA9) and 2,3-dehydroGA9 to GA7; GA9 was also metabolized to GA4 in a branch pathway. The preparation from Marah seeds also metabolized GA5 to GA3 in high yield; GA6 was a minor product and was not metabolized to GA3. Using substrates stereospecifically labeled with deuterium, it was shown that the metabolism of GA5 to GA3 and of 2,3-dehydroGA9 to GA7 occurs with the loss of the 1{beta}-hydrogen. In cultures of Gibberella fujikuroi, mutant B1-41a, [1{beta},2{beta}-2H2]GA4, was metabolized to [1,2-2H2]GA3 with the loss of the 1{alpha}- and 2{alpha}-hydrogens. These results provide further evidence that the biosynthetic origin of GA3 and GA7 in higher plants is different from that in the fungus Gibberella fujikuroi.

1 Financial support is gratefully acknowledged from the Agricultural and Food Research Council (J.MacM.) and the National Science Foundation, grant DMB-8506998 (B.O.P.).




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