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Plant Physiology 94:245-250 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Activation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) by Rubisco Activase 1

Effects of Some Sugar Phosphates

Ross McC. Lilley2 and Archie R. Portis, Jr.

U.S. Department of Agriculture, Agricultural Research Service, 1102 S. Goodwin Avenue, Urbana, Illinois 61801, Photosynthesis Research Unit, 1102 S. Goodwin Avenue, Urbana, Illinois 61801

The activation of purified ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) has been studied in the presence of sugar phosphates, and the effect of rubisco activase on this process determined. During an 11-minute time course at pH 7.7 and 11 micromolar CO2, the activation of rubisco was strongly inhibited by ribulose-1,5-bisphosphate (4 millimolar), fructose-1,6-bisphosphate (1 millimolar) and ribose 5-phosphate (5 millimolar), but this inhibition was overcome by the addition of rubisco activase and activation then proceeded to a greater extent than spontaneous activation of rubisco. Glycerate 3-phosphate (20 millomolar) slowed the initial rate but not the extent of activation and rubisco activase had no effect on this. The activation of rubisco was shown to be affected by phosphoenolpyruvate (3 millimolar) but not by creatine phosphate (3 millimolar) or ATP (3 millimolar), and the creatine-phosphate/creatine phosphokinase system was used to generate the high ATP/ADP quotients required for rubisco activase to function. ATP was shown to be required for the rubisco activase-dependent rubisco activation in the presence of fructose-1,6-bisphosphate (1 millimolar). It is concluded that rubisco activase has a mixed specificity for some sugar phosphate-bound forms of rubisco, but has low or no activity with others. Some possible bases for these differences among sugar phosphates are discussed but remain to be established.

2 Permanent address: Department of Biology, University of Wollongong, P. O. Box 1144 Wollongong, N.S.W. 2500, Australia.

1 Supported in part by Grant 88-37234-3383 of the Competitive Research Grants Program of the U.S. Department of Agriculture.




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L. Hendrickson, R. Sharwood, M. Ludwig, S. M. Whitney, M. R. Badger, and S. von Caemmerer
The effects of Rubisco activase on C4 photosynthesis and metabolism at high temperature
J. Exp. Bot., May 1, 2008; 59(7): 1789 - 1798.
[Abstract] [Full Text] [PDF]


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