Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 94:268-277 (1990)
© 1990 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Z.-C.
Right arrow Articles by Bush, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Z.-C.
Right arrow Articles by Bush, D. R.
Agricola
Right arrow Articles by Li, Z.-C.
Right arrow Articles by Bush, D. R.
Membranes and Bioenergetics

{Delta}pH-Dependent Amino Acid Transport into Plasma Membrane Vesicles Isolated from Sugar Beet Leaves

I. Evidence for Carrier-Mediated, Electrogenic Flux through Multiple Transport Systems

Zhen-Chang Li and Daniel R. Bush

Photosynthesis Research Unit. U.S. Department of Agriculture, Agricultural Research Service, University of Illinois, Urbana, Illinois 61801, Department of Plant Biology, University of Illinois, Urbana, Illinois 61801

Amino acid transport into plasma membrane vesicles isolated from mature sugar beet (Beta vulgaris L. cv Great Western) leaves was investigated. The transport of alanine, leucine, glutamine, glutamate, isoleucine, and arginine was driven by a trans-membrane proton concentration difference. {Delta}pH-Dependent alanine, leucine, glutamine, and glutamate transport exhibited simple Michaelis-Menten kinetics, and double-reciprocal plots of the data were linear with apparent Km values of 272, 346, 258, and 1981 micromolar, respectively. These results are consistent with carrier mediated transport. {Delta}pH-Dependent isoleucine and arginine transport exhibited biphasic kinetics, suggesting these amino acids may be transported by at least two transport systems. Symport mediated alanine transport was electrogenic as demonstrated by the effect of membrane potential ({Delta}{Psi}) on {Delta}pH-dependent flux. In the absence of significant charge compensation, a low rate of alanine transport was observed. When {Delta}{Psi} was held at 0 millivolt with symmetric potassium concentrations and valinomycin, the rate of flux was stimulated fourfold. In the presence of a negative {Delta}{Psi}, alanine transport increased sixfold. These results are consistent with an electrogenic transport process which results in a net flux of positive charge into the vesicles. The effect of changing {Delta}{Psi} on the kinetics of alanine transport altered Vmax with no apparent change in Km. Amino acid transport was inhibited by the protein modifier diethyl pyrocarbonate, but was insensitive to N-ethylmaleimide, 4,4'-diisothiocyano-2,2'-stilbene disulfonic acid, p-chloromercuribenzenesulfonic acid, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide. Four amino acid symport systems, two neutral, one acidic, and one basic, were resolved based on inter-amino acid competition experiments. One neutral system appears to be active for all neutral amino acids while the second exhibited a low affinity for isoleucine, threonine, valine, and proline. Although each symport was relatively specific for a given group of amino acids, each system exhibited some crossover specificity for amino acids in other groups.




This article has been cited by other articles:


Home page
 Plant CellHome page
A. Hirner, F. Ladwig, H. Stransky, S. Okumoto, M. Keinath, A. Harms, W. B. Frommer, and W. Koch
Arabidopsis LHT1 Is a High-Affinity Transporter for Cellular Amino Acid Uptake in Both Root Epidermis and Leaf Mesophyll
PLANT CELL, August 1, 2006; 18(8): 1931 - 1946.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Bae, M. S. Kim, R. C. Sicher, H.-J. Bae, and B. A. Bailey
Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis
Plant Physiology, July 1, 2006; 141(3): 1056 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Okumoto, W. Koch, M. Tegeder, W. N. Fischer, A. Biehl, D. Leister, Y. D. Stierhof, and W. B. Frommer
Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3
J. Exp. Bot., October 1, 2004; 55(406): 2155 - 2168.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Bourque, R. Lemoine, A. Sequeira-Legrand, L. Fayolle, S. Delrot, and A. Pugin
The Elicitor Cryptogein Blocks Glucose Transport in Tobacco Cells
Plant Physiology, December 1, 2002; 130(4): 2177 - 2187.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Okumoto, R. Schmidt, M. Tegeder, W. N. Fischer, D. Rentsch, W. B. Frommer, and W. Koch
High Affinity Amino Acid Transporters Specifically Expressed in Xylem Parenchyma and Developing Seeds of Arabidopsis
J. Biol. Chem., November 15, 2002; 277(47): 45338 - 45346.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Delétage-Grandon, J.-F. Chollet, M. Faucher, F. Rocher, E. Komor, and J.-L. Bonnemain
Carrier-Mediated Uptake and Phloem Systemy of a 350-Dalton Chlorinated Xenobiotic with an {alpha}-Amino Acid Function
Plant Physiology, April 1, 2001; 125(4): 1620 - 1632.
[Abstract] [Full Text]

Home page
 Plant Cell PhysiolHome page
A. C. Borstlap and J. A.M.J. Schuurmans
Proton-Symport of L-Valine in Plasma Membrane Vesicles Isolated from Leaves of the Wild-Type and the Valr-2 Mutant of Nicotiana tabacum L.
Plant Cell Physiol., November 1, 2000; 41(11): 1210 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Tegeder, C. E. Offler, W. B. Frommer, and J. W. Patrick
Amino Acid Transporters Are Localized to Transfer Cells of Developing Pea Seeds
Plant Physiology, February 1, 2000; 122(2): 319 - 326.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
H. U. Kosegarten, B. Hoffmann, and K. Mengel
Apoplastic pH and Fe3+ Reduction in Intact Sunflower Leaves
Plant Physiology, December 1, 1999; 121(4): 1069 - 1079.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
A. Neelam, A. C. Marvier, J.L. Hall, and L. E. Williams
Functional Characterization and Expression Analysis of the Amino Acid Permease RcAAP3 from Castor Bean
Plant Physiology, August 1, 1999; 120(4): 1049 - 1056.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H.-C. Chang and D. R. Bush
Topology of NAT2, a Prototypical Example of a New Family of Amino Acid Transporters
J. Biol. Chem., November 28, 1997; 272(48): 30552 - 30557.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Boorer and W.-N. Fischer
Specificity and Stoichiometry of the Arabidopsis H+/Amino Acid Transporter AAP5
J. Biol. Chem., May 16, 1997; 272(20): 13040 - 13046.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Boorer, W. B. Frommer, D. R. Bush, M. Kreman, D. D. F. Loo, and E. M. Wright
Kinetics and Specificity of a H[IMAGE]/Amino Acid Transporter from Arabidopsis thaliana
J. Biol. Chem., January 26, 1996; 271(4): 2213 - 2220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W.-N. Fischer, M. Kwart, S. Hummel, and W. B. Frommer
Substrate Specificity and Expression Profile of Amino Acid Transporters (AAPs) in Arabidopsis
J. Biol. Chem., July 7, 1995; 270(27): 16315 - 16320.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1990 by the American Society of Plant Biologists