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Plant Physiology 94:492-498 (1990)
© 1990 American Society of Plant Biologists

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Metabolism and Enzymology

Biosynthesis of Triacylglycerols Containing Very Long Chain Monounsaturated Acyl Moieties in Developing Seeds 1

Eberhard Fehling, Denis J. Murphy2 and Kumar D. Mukherjee

Federal Center for Lipid Research, Institute for Biochemistry and Technology, -H.P. Kaufmann-Institute-, Piusallee 68, D-4400 Münster, Federal Republic of Germany

Particulate (15,000g) fractions from developing seeds of honesty (Lunaria annua L.) and mustard (Sinapis alba L.) synthesize radioactive very long chain monounsaturated fatty acids (gadoleic, erucic, and nervonic) from [1-14C]oleoyl-CoA and malonyl-CoA or from oleoyl-CoA and [2-14C]malonyl-CoA. The very long chain monounsaturated fatty acids are rapidly channeled to triacylglycerois and other acyl lipids without intermediate accumulation of their CoA thioesters. When [1-14C]oleoyl-CoA is used as the radioactive substrate, phosphatidylcholines and other phospholipids are most extensively radiolabeled by oleoyl moieties rather than by very long chain monounsaturated acyl moieties. When [2-14C]malonyl-CoA is used as the radioactive substrate, no radioactive oleic acid is formed and the newly synthesized very long chain monounsaturated fatty acids are extensively incorporated into phosphatidylcholines and other phospholipids as well as triacylglycerols. The pattern of labeling of the key intermediates of the Kennedy pathway, e.g. lysophosphatidic acids, phosphatidic acids, and diacylglycerols by the newly synthesized very long chain monounsaturated fatty acids is consistent with the operation of this pathway in the biosynthesis of triacylglycerols.

2 Permanent address: Department of Brassica and Oilseeds Research, Cambridge Laboratory, John Innes Centre for Plant Science Research, Colney Lane, Norwich NR4 7UJ, United Kingdom.

1 Part of this work was supported by a grant from Bundesministerium für Forschung und Technologie (BMFT), Bonn, Federal Republic of Germany. One of the authors (D. J. M.) is also supported by the Agriculture and Food Research Council, United Kingdom.






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Copyright © 1990 by the American Society of Plant Biologists