Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 94:1237-1243 (1990)
© 1990 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bailey-Serres, J.
Right arrow Articles by Freeling, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bailey-Serres, J.
Right arrow Articles by Freeling, M.
Agricola
Right arrow Articles by Bailey-Serres, J.
Right arrow Articles by Freeling, M.
Environmental and Stress Physiology

Hypoxic Stress-Induced Changes in Ribosomes of Maize Seedling Roots 1

Julia Bailey-Serres2 and Michael Freeling

Department of Plant Biology, University of California, Berkeley, California, 94720

The hypoxic stress response of Zea mays L. seedling roots involves regulation of gene expression at transcriptional and posttranscriptional levels. We investigated the effect of hypoxia on the translational machinery of seedling roots. The levels of monoribosomes and ribosomal subunits increased dramatically within 1 hour of stress. Prolonged hypoxia resulted in continued accumulation of nontranslating ribosomes, as well as increased levels of small polyribosomes. The return of seedlings to normal aerobic conditions resulted in recovery of normal polyribosome levels. Comparison of ribosomal proteins from control and hypoxic roots revealed differences in quantity and electrophoretic mobility. In vivo labeling of roots with [35S]methionine revealed variations in newly synthesized ribosomal proteins. In vivo labeling of roots with [32P]orthophosphate revealed a major reduction in the phosphorylation of a 31 kilodalton ribosomal protein in hypoxic stressed roots. In vitro phosphorylation of ribosomal proteins by endogenous kinases was used to probe for differences in ribosome structure and composition. The patterns of in vitro kinased phosphoproteins of ribosomes from control and hypoxic roots were not identical. Variation in phosphoproteins of polyribosomes from control and hypoxic roots, as well as among polyribosomes from hypoxic roots were observed. These results indicate that modification of the translational machinery occurs in response to hypoxic stress.

2 Present address: Department of Botany and Plant Sciences, University of California, Riverside, CA 92521.

1 Supported by postdoctoral research awards from National Institutes of Health (5-F32-GM11112-03) and U.S. Department of Agriculture (89-37264-4837) to J.B.-S. and a grant from National Institutes of Health grant (2-R01-GM21734-12) to M.F.




This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
A. J. Carroll, J. L. Heazlewood, J. Ito, and A. H. Millar
Analysis of the Arabidopsis Cytosolic Ribosome Proteome Provides Detailed Insights into Its Components and Their Post-translational Modification
Mol. Cell. Proteomics, February 1, 2008; 7(2): 347 - 369.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. AGARWAL and A. GROVER
Isolation and Transcription Profiling of Low-O2 Stress-Associated cDNA Clones from the Flooding-stress-tolerant FR13A Rice Genotype
Ann. Bot., October 1, 2005; 96(5): 831 - 844.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
C. BRANCO-PRICE, R. KAWAGUCHI, R. B. FERREIRA, and J. BAILEY-SERRES
Genome-wide Analysis of Transcript Abundance and Translation in Arabidopsis Seedlings Subjected to Oxygen Deprivation
Ann. Bot., September 1, 2005; 96(4): 647 - 660.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I.-F. Chang, K. Szick-Miranda, S. Pan, and J. Bailey-Serres
Proteomic Characterization of Evolutionarily Conserved and Variable Proteins of Arabidopsis Cytosolic Ribosomes
Plant Physiology, March 1, 2005; 137(3): 848 - 862.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Casati and V. Walbot
Crosslinking of Ribosomal Proteins to RNA in Maize Ribosomes by UV-B and Its Effects on Translation
Plant Physiology, October 1, 2004; 136(2): 3319 - 3332.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J. Williams, J. Werner-Fraczek, I.-F. Chang, and J. Bailey-Serres
Regulated Phosphorylation of 40S Ribosomal Protein S6 in Root Tips of Maize
Plant Physiology, August 1, 2003; 132(4): 2086 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
C. C. SUBBAIAH and M. M. SACHS
Molecular and Cellular Adaptations of Maize to Flooding Stress
Ann. Bot., January 2, 2003; 91(2): 119 - 127.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
R. HANSCH, R. R. MENDEL, R. CERFF, and R. HEHL
Light-dependent Anaerobic Induction of the Maize Glyceraldehyde-3-Phosphate Dehydrogenase 4 (GapC4) Promoter in Arabidopsis thaliana and Nicotiana tabacum
Ann. Bot., January 2, 2003; 91(2): 149 - 154.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. J. Klok, I. W. Wilson, D. Wilson, S. C. Chapman, R. M. Ewing, S. C. Somerville, W. J. Peacock, R. Dolferus, and E. S. Dennis
Expression Profile Analysis of the Low-Oxygen Response in Arabidopsis Root Cultures
PLANT CELL, October 1, 2002; 14(10): 2481 - 2494.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E.S. Dennis, R. Dolferus, M. Ellis, M. Rahman, Y. Wu, F.U. Hoeren, A. Grover, K.P. Ismond, A.G. Good, and W.J. Peacock
Molecular strategies for improving waterlogging tolerance in plants
J. Exp. Bot., January 1, 2000; 51(342): 89 - 97.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Zeng, Y. Wu, W. T. Avigne, and K. E. Koch
Rapid Repression of Maize Invertases by Low Oxygen. Invertase/Sucrose Synthase Balance, Sugar Signaling Potential, and Seedling Survival
Plant Physiology, October 1, 1999; 121(2): 599 - 608.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
J.-Y. Cho and T. W. Jeffries
Transcriptional Control of ADH Genes in the Xylose-Fermenting Yeast Pichia stipitis
Appl. Envir. Microbiol., June 1, 1999; 65(6): 2363 - 2368.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. Manjunath, C.-H. Kenneth Lee, P. V. Winkle, and J. Bailey-Serres
Molecular and Biochemical Characterization of Cytosolic Phosphoglucomutase in Maize . Expression during Development and in Response to Oxygen Deprivation
Plant Physiology, July 1, 1998; 117(3): 997 - 1006.
[Abstract] [Full Text]

Home page
 GeneticsHome page
F. U. Hoeren, R. Dolferus, Y. Wu, W. J. Peacock, and E. S. Dennis
Evidence for a Role for AtMYB2 in the Induction of the Arabidopsis Alcohol Dehydrogenase Gene (ADH1) Low Oxygen
Genetics, June 1, 1998; 149(2): 479 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. K. Morelli, W. Zhou, J. Yu, C. Lu, and M. E. Vayda
Actin Depolymerization Affects Stress-Induced Translational Activity of Potato Tuber Tissue
Plant Physiology, April 1, 1998; 116(4): 1227 - 1237.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1990 by the American Society of Plant Biologists