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Plant Physiology 94:1444-1448 (1990)
© 1990 American Society of Plant Biologists

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Microbe-Plant Interactions

Biosynthesis of the Phytoalexin Pisatin 1

Isoflavone Reduction and Further Metabolism of the Product Sophorol by Extracts of Pisum sativum

Carol L. Preisig2, John N. Bell3, Yuejin Sun, Geza Hrazdina, David E. Matthews4 and Hans D. VanEtten5

Department of Plant Pathology, Cornell University, Ithaca, New York 14853, Department of Food Science and Technology, New York State Agriculture Experiment Station, Cornell University, Geneva, New York 14856

NADPH-dependent reduction of 2',7-dihydroxy-4',5'-methylenedioxyisoflavone to the isoflavanone sophorol, a proposed intermediate step in pisatin biosynthesis, was detected in extracts of Pisum sativum. This isoflavone reductase activity was inducible by treatment of pea seedlings with CuCl2. The timing of induction coincided with that of the 6a-hydroxymaackiain 3-O-methyltransferase, which catalyzes the terminal biosynthetic step. Neither enzyme was light inducible. Further NADPH-dependent metabolism of sophorol by extracts of Cucl2-treated seedlings was also observed; three products were radiolabeled when [3H]sophorol was the substrate, one of which is tentatively identified as maackiain.

2 Present address: U.S. Department of Agriculture Eastern Regional Research Center, 600 East Mermaid Lane, Philadelphia, PA 19118.

3 Present address: Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44166.

4 Present address: Department of Plant Breeding, Cornell University, Ithaca, NY 14853.

5 Present address: Department of Plant Pathology, University of Arizona, Tucson, AZ 85721.

1 This material is based upon work supported by the National Science Foundation under grants No. DMB85-05697 and DCB-8916265.






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Copyright © 1990 by the American Society of Plant Biologists