Plant Physiol.
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Plant Physiology 94:970-979 (1990)
© 1990 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

Stable Accumulation of Modified 2S Albumin Seed Storage Proteins with Higher Methionine Contents in Transgenic Plants 1

Ann De Clercq, Martine Vandewiele, Jozef Van Damme, Philippe Guerche2, Marc Van Montagu, Joël Vandekerckhove and Enno Krebbers

Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium, Plant Genetic Systems N.V., Plateaustraat 22, B-9000 Gent, Belgium

We present the results of two sets of experiments designed to express high methionine proteins in transgenic seeds in three different plant species. In the first approach, two chimeric genes were constructed in which parts of the Arabidopsis 2S albumin gene 1 (AT2S1) were fused at different positions to a Brazil nut 2S albumin cDNA clone. Brazil nut 2S albumin was found to accumulate stably in transgenic Arabidopsis, Brassica napus, and tobacco seeds. In the second approach, methionine-enriched AT2S1 genes were constructed by deleting sequences encoding a region of the protein which is not highly conserved among 2S albumins of different species and replacing them with methioninerich sequences. Introduction of the modified AT2S1 genes into three different plant species resulted in the accumulation of the methionine-enriched 2S albumins in all three species at levels reaching 1 to 2% of the total high salt-extractable seed protein.

2 Present address: Laboratoire de Biologie Cellulaire INRA, Centre de Versailles, Route de Saint-Cyr, F-78026 Versailles Cedex, France.

1 A.D.C. is indebted to the Instituut tot Aanmoediging van het Wetenschappelijk Onderzoek in Nijverheid en Landbouw for a predoctoral fellowship, and J.V. was a Research Associate of the National Fund for Scientific Research (Belgium).




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A. Goossens, W. Dillen, J. De Clercq, M. Van Montagu, and G. Angenon
The arcelin-5 Gene of Phaseolus vulgaris Directs High Seed-Specific Expression in Transgenic Phaseolus acutifolius and Arabidopsis Plants
Plant Physiology, August 1, 1999; 120(4): 1095 - 1104.
[Abstract] [Full Text]


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