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Molecular Biology and Gene Regulation

Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene ¹

Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan, Department of Botany, Faculty of Science, Hokkaido University, Sapporo 604, Japan

We obtained carrot (Daucus carota) cells possessing the 5'-noncoding sequence of the ORF12 gene (roIC) of TL-DNA of the Ri plasmid and a structural gene of bacterial -glucuronidase by Agrobacterium-mediated transformation. When such cells were cultured in medium containing 2,4-dichlorophenoxyacetic acid, substantial reduction in -glucuronidase activity was observed. Upon transferring the cells from a 2,4-D-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expression of -glucuronidase in somatic embryo development was recorded. Activation by gibberillic acid and suppression by abscisic acid of -glucuronidase activities, in concord with embryogenesis, were also noted.