Plant Physiol. Drug Metab Dispos
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Plant Physiology 95:687-692 (1991)
© 1991 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

Expression of DNA Coding for Diphtheria Toxin Chain A Is Toxic to Plant Cells 1

Mihaly Czako and Gynheung An

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340

DNA coding for the enzymatically active subunit A of diphtheria toxin was placed under the control of the cauliflower mosaic virus 35S promoter and the Agrobacterium left transfer-DNA gene 7 polyadenylation signal. Agrobacteria carrying a binary plant vector with the chimeric diphtheria toxin A gene had very low transforming activity in tobacco (Nicotiana tabacum L.), and greatly diminished the recovery of stable transformants when mixed together with agrobacteria which alone transformed plant cells well. The introduction of this chimeric molecule into tobacco cells by electroporation lowered the level of the transient expression of the coelectroporated chloramphenicol acetyltransferase reporter gene indicating that expression of diphtheria toxin chain A in plant cells is toxic. We have developed a binary vector pGA987 which can be used for probing a variety of plant promoters.

1 This work was supported in part by grants from Washington State University and the Rockefeller Foundation.




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[Abstract] [Full Text]


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Copyright © 1991 by the American Society of Plant Biologists