This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (59) Citing Articles via Google Scholar Google Scholar Articles by Christie, P. J. Articles by Walbot, V. Search for Related Content PubMed PubMed Citation Articles by Christie, P. J. Articles by Walbot, V. Agricola Articles by Christie, P. J. Articles by Walbot, V.

Environmental and Stress Physiology

Low-Temperature Accumulation of Alcohol Dehydrogenase-1 mRNA and Protein Activity in Maize and Rice Seedlings ¹

Department of Biological Sciences, Stanford University, Stanford, California 94305-5020

Low-temperature stress was shown to cause a rapid increase in steady-state levels of alcohol dehydrogenase-1 message (Adh1) and protein activity (ADH1) in maize (Zea mays) (B37N, A188) and rice (Oryza sativa) (Taipei 309, Calmochi 101) seedlings. Maize roots and rice shoots and roots from 7-day seedlings shifted to low temperature (10°C) contained as much as 15-fold more Adh1 mRNA and 8-fold more ADH1 protein activity than the corresponding tissues from untreated seedlings. Time-course studies showed that these tissues accumulated Adh1 mRNA and ADH1 activity severalfold within 4- to 8-hour, levels plateaued within 20 to 24 hours, and remained elevated at 4 days of cold treatment. Within 24 hours of returning cold-stressed seedlings to ambient temperature, Adh1 mRNA and ADH1 activity decreased to pretreatment levels. Histochemical staining of maize and rice tissue imprints showed that ADH activity was enhanced along the lengths of cold-stressed maize primary roots and rice roots, and along the stems and leaves of rice shoots. Our observations suggest that short-term cold stress induces Adh1 gene expression in certain plant tissues, which, reminiscient of the anaerobic response, may reflect a fundamental shift in energy metabolism to ensure tissue survival during the stress period.

¹ Supported by the Rockefeller Foundation (grant RF 87058/#58). P.J.C. was supported in part by American Cancer Society grant PF2937. M.H. was supported by postdoctoral fellowships from the Deutsche Forschungsgemeinschaft and the Deutscher Akademischer Austauschdienst (Sonderprogramm Gentechnologie).

This article has been cited by other articles:

				R. Vidal, L. Lopez-Maury, M. G. Guerrero, and F. J. Florencio Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System J. Bacteriol., July 1, 2009; 191(13): 4383 - 4391. [Abstract] [Full Text] [PDF]

				C. Tesniere, L. Torregrosa, M. Pradal, J.-M. Souquet, C. Gilles, K. Dos Santos, P. Chatelet, and Z. Gunata Effects of genetic manipulation of alcohol dehydrogenase levels on the response to stress and the synthesis of secondary metabolites in grapevine leaves J. Exp. Bot., January 1, 2006; 57(1): 91 - 99. [Abstract] [Full Text] [PDF]

				C. Tesniere, M. Pradal, A. El-Kereamy, L. Torregrosa, P. Chatelet, J.-P. Roustan, and C. Chervin Involvement of ethylene signalling in a non-climacteric fruit: new elements regarding the regulation of ADH expression in grapevine J. Exp. Bot., October 1, 2004; 55(406): 2235 - 2240. [Abstract] [Full Text] [PDF]

				O. Kursteiner, I. Dupuis, and C. Kuhlemeier The Pyruvate decarboxylase1 Gene of Arabidopsis Is Required during Anoxia But Not Other Environmental Stresses Plant Physiology, June 1, 2003; 132(2): 968 - 978. [Abstract] [Full Text] [PDF]