Plant Physiology 95:760-767 (1991)
© 1991 American Society of Plant Biologists
Environmental and Stress Physiology
Response to Anoxia in Rice and Wheat Seedlings
Changes in the pH of Intracellular Compartments, Glucose-6-Phosphate Level, and Metabolic Rate
Faustino Menegus,
Liliana Cattaruzza,
Monica Mattana,
Nicoletta Beffagna and
Enzio Ragg
Istituto Biosintesi Vegetali, CNR, Via Bassini 15, 20133 Milano, Italy,
Centro per lo studio sulla Biologia Cellulare e Molecolare delle Piante, Via Celoria 26, 20133 Milano, Italy,
Dipartimento di Scienze Molecolari Agroalimentari, Facoltà di Agraria, Via Celoria 2, 20133 Milano, Italy
31P nuclear magnetic resonance spectroscopy was used to measure intracellular pH in living tissues. Oxygen deprivation caused fast cytoplasmic acidification from pH 7.4 to 7.0 in shoots of rice, Oryza sativa L. var arborio, a species highly resistant to anoxia. Acidification was complete after 10 minutes of anoxia. Alkalinization of both cytosplasm and vacuole followed thereafter. In the anoxia intolerant wheat shoots, Triticum aestivum L. var MEK, the same treatment caused a sharper cytoplasmic acidification, from pH 7.4 to 6.6, which occurred during a period of 2 hours. Cytoplasmic acidification continued with progress of anoxia and there was no vacuolar alkalinization comparable to the one observed in rice. In wheat oxyen, withdrawal also caused the reduction of both glucose-6-phosphate level and of metabolic rate. It also induced heavy losses of inorganic phosphate from tissues. Conversely, in rice, glucose-6-phosphate level and metabolic rate were increased and inorganic phosphate leakage from tissues was completely absent. These results are discussed in relation to the mechanisms of plant resistance to anoxia.
This article has been cited by other articles:
|
|
|
|
 
R. Narsai, K. A. Howell, A. Carroll, A. Ivanova, A. H. Millar, and J. Whelan
Defining Core Metabolic and Transcriptomic Responses to Oxygen Availability in Rice Embryos and Young Seedlings
Plant Physiology,
September 1, 2009;
151(1):
306 - 322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matthew, W. Zhou, J. Rupprecht, L. Lim, S. R. Thomas-Hall, A. Doebbe, O. Kruse, B. Hankamer, U. C. Marx, S. M. Smith, et al.
The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H2 Production by Sulfur Depletion
J. Biol. Chem.,
August 28, 2009;
284(35):
23415 - 23425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Kulichikhin, H. Greenway, L. Byrne, and T. D. Colmer
Regulation of intracellular pH during anoxia in rice coleoptiles in acidic and near neutral conditions
J. Exp. Bot.,
May 1, 2009;
60(7):
2119 - 2128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Magneschi and P. Perata
Rice germination and seedling growth in the absence of oxygen
Ann. Bot.,
January 1, 2009;
103(2):
181 - 196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. H. FELLE
Apoplastic pH During Low-oxygen Stress in Barley
Ann. Bot.,
November 1, 2006;
98(5):
1085 - 1093.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. HUANG, H. GREENWAY, T. D. COLMER, and A. H. MILLAR
Protein Synthesis by Rice Coleoptiles During Prolonged Anoxia: Implications for Glycolysis, Growth and Energy Utilization
Ann. Bot.,
September 1, 2005;
96(4):
703 - 715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Huang, K. Ishizawa, H. Greenway, and T. D. Colmer
Manipulation of ethanol production in anoxic rice coleoptiles by exogenous glucose determines rates of ion fluxes and provides estimates of energy requirements for cell maintenance during anoxia
J. Exp. Bot.,
September 1, 2005;
56(419):
2453 - 2463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Huang, H. Greenway, and T. D. Colmer
Anoxia tolerance in rice seedlings: exogenous glucose improves growth of an anoxia-'intolerant', but not of a 'tolerant' genotype
J. Exp. Bot.,
October 1, 2003;
54(391):
2363 - 2373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. HUANG, H. GREENWAY, and T. D. COLMER
Responses by Coleoptiles of Intact Rice Seedlings to Anoxia: K+ Net Uptake from the External Solution and Translocation from the Caryopses
Ann. Bot.,
January 2, 2003;
91(2):
271 - 278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. D. Colmer, S. Huang, and H. Greenway
Evidence for down-regulation of ethanolic fermentation and K+ effluxes in the coleoptile of rice seedlings during prolonged anoxia
J. Exp. Bot.,
July 1, 2001;
52(360):
1507 - 1517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Gout, A.-M. Boisson, S. Aubert, R. Douce, and R. Bligny
Origin of the Cytoplasmic pH Changes during Anaerobic Stress in Higher Plant Cells. Carbon-13 and Phosphorous-31 Nuclear Magnetic Resonance Studies
Plant Physiology,
February 1, 2001;
125(2):
912 - 925.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. E. Summers, R. G. Ratcliffe, and M. B. Jackson
Anoxia tolerance in the aquatic monocot Potamogeton pectinatus: absence of oxygen stimulates elongation in association with an unusually large Pasteur effect
J. Exp. Bot.,
August 1, 2000;
51(349):
1413 - 1422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Gibbs, S. Morrell, A Valdez, T.L Setter, and H. Greenway
Regulation of alcoholic fermentation in coleoptiles of two rice cultivars differing in tolerance to anoxia
J. Exp. Bot.,
April 1, 2000;
51(345):
785 - 796.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Kronzucker, G. J.D. Kirk, M. Yaeesh Siddiqi, and A. D.M. Glass
Effects of Hypoxia on 13NH4+ Fluxes in Rice Roots . Kinetics and Compartmental Analysis
Plant Physiology,
February 1, 1998;
116(2):
581 - 587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
