This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (54) Citing Articles via Google Scholar Google Scholar Articles by Tucker, M. L. Articles by Milligan, S. B. Search for Related Content PubMed PubMed Citation Articles by Tucker, M. L. Articles by Milligan, S. B. Agricola Articles by Tucker, M. L. Articles by Milligan, S. B.

Molecular Biology and Gene Regulation

Sequence Analysis and Comparison of Avocado Fruit and Bean Abscission Cellulases ^1,2,3

U.S. Department of Agriculture, Agricultural Research Service, Plant Molecular Biology Laboratory, Room 207, Building 006, BARC-West, Beltsville, Maryland 20705

A 1700 nucleotide cDNA clone for a bean (Phaseolus vulgaris cv Red Kidney) abscission cellulase (endo-(1,4)--D-glucanase) has been identified and sequenced. This cDNA clone contains a 1485 nucleotide open reading frame which includes coding sequences for a putative signal peptide and mature protein. The nucleotide and deduced amino acid sequences for the bean abscission cellulase are compared to the previously reported sequences of an avocado fruit ripening cellulase. Optimal alignment of these sequences shows 64% and 50% identically matched nucleotides and amino acids, respectively. Analysis of the deduced amino acid sequences for the mature bean and avocado cellulases indicates that these two proteins share similar molecular weights, position of cysteine residues, and hydropathic character, but have very different isoelectric points and glycosylation. Genomic blot data suggest that the avocado fruit cellulase belongs to a small gene family, whereas the bean abscission cellulase appears to be encoded by a single gene or a few very closely related genes.

¹ Supported in part by National Science Foundation grant DCB-8310460 and U.S. Department of Agriculture grant 8900650.

² In memory of Dr. Jacob B. Biale.

³ The nucleotide sequence of the bean abscission cellulase cDNA has been deposited in the GenBank sequence library, accession number M57400.

This article has been cited by other articles:

				J. Clements and C. Atkins Characterization of a non-abscission mutant in Lupinus angustifolius. I. Genetic and structural aspects Am. J. Botany, January 1, 2001; 88(1): 31 - 42. [Abstract] [Full Text]

				I. Llop-Tous, E. Domínguez-Puigjaner, X. Palomer, and M. Vendrell Characterization of Two Divergent Endo-beta -1,4-Glucanase cDNA Clones Highly Expressed in the Nonclimacteric Strawberry Fruit Plant Physiology, April 1, 1999; 119(4): 1415 - 1422. [Abstract] [Full Text]

				C. A. Loopstra, A. Mouradov, A. Vivian-Smith, T. V. Glassick, B. V. Gale, S. G. Southerton, H. Marshall, and R. D. Teasdale Two Pine Endo-beta -1,4-Glucanases Are Associated with Rapidly Growing Reproductive Structures Plant Physiology, March 1, 1998; 116(3): 959 - 967. [Abstract] [Full Text]