This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gillikin, J. W. Articles by Graham, J. S. Search for Related Content PubMed PubMed Citation Articles by Gillikin, J. W. Articles by Graham, J. S. Agricola Articles by Gillikin, J. W. Articles by Graham, J. S.

Metabolism and Enzymology

Purification and Developmental Analysis of the Major Anionic Peroxidase from the Seed Coat of Glycine max ¹

Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403

We show that the majority of peroxidase activity in soybean (Glycine max var Williams 82) seeds is localized to the seed coat. A single isozyme is responsible for this activity and has been purified to electrophoretic homogeneity by successive chromatography on DEAE Sepharose Fast Flow, concanavalin A-Sepharose, and Sephadex G-75. The peroxidase exhibits a pl of 4.1, an apparent molecular mass of 37 kilodaltons, and has properties characteristic of a glycoprotein. The enzyme begins to accumulate approximately 21 days after anthesis and continues to do so throughout the maturation of the seed coat where it can represent at least 5% of the soluble protein in dry seed coats. Due to its localization in the seed, we propose that this isozyme may play a role in the hardening of the seed coat.

This article has been cited by other articles:

				A. B. Downie, L. M. A. Dirk, Q. Xu, J. Drake, D. Zhang, M. Dutt, A. Butterfield, R. R. Geneve, J. W. Corum III, K. G. Lindstrom, et al. A physical, enzymatic, and genetic characterization of perturbations in the seeds of the brownseed tomato mutants J. Exp. Bot., May 1, 2004; 55(399): 961 - 973. [Abstract] [Full Text] [PDF]

				A. B. Downie, D. Zhang, L. M.A. Dirk, R. R. Thacker, J. A. Pfeiffer, J. L. Drake, A. A. Levy, D. A. Butterfield, J. W. Buxton, and J. C. Snyder Communication between the Maternal Testa and the Embryo and/or Endosperm Affect Testa Attributes in Tomato Plant Physiology, September 1, 2003; 133(1): 145 - 160. [Abstract] [Full Text] [PDF]

				A. K. JanardhananPillai Kizhakkedathu, M. Nazeerunnisa, and D. V. Behere Thermal Unfolding of Soybean Peroxidase. APPROPRIATE HIGH DENATURANT CONCENTRATIONS INDUCE COOPERATIVITY ALLOWING THE CORRECT MEASUREMENT OF THERMODYNAMIC PARAMETERS J. Biol. Chem., October 18, 2002; 277(43): 40717 - 40721. [Abstract] [Full Text] [PDF]