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Cellular and Structural Biology

Chloroplast Structure and Function Is Altered in the NCS2 Maize Mitochondrial Mutant ¹

Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, Department of Forestry, University of Missouri, Columbia, Missouri 65211

The nonchromosomal stripe 2 (NCS2) mutant of maize (Zea mays L.) has a DNA rearrangement in the mitochondrial genome that segregates with the abnormal growth phenotype. Yet, the NCS2 characteristic phenotype includes striped sectors of pale-green tissue on the leaves. This suggests a chloroplast abnormality. To characterize the chloroplasts present in the mutant sectors, we examined the chloroplast structure by electron microscopy, chloroplast function by radiolabeled carbon dioxide fixation and fluorescence induction kinetics, and thylakoid protein composition by polyacrylamide gel electrophoresis. The data from these analyses suggest abnormal or prematurely arrested chloroplast development. Deleterious effects of the NCS2 mutant mitochondria upon the cells of the leaf include structural and functional alterations in the both the bundle sheath and mesophyll chloroplasts.

¹ Supported by grant DCB885827 from the National Science Foundation and gift funds from Pioneer Hi-Bred Int. to K. J. N.

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