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Metabolism and Enzymology

Fructosyl Transfer between 1-Kestose and Sucrose in Wheat Leaves ¹

Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907

The labeling pattern of the sugar moieties of 1-kestose after in vivo pulse labeling with ¹⁴CO₂ was not the same as that after in vitro labeling with ¹⁴C-sucrose. The two fructosyl residues of 1-kestose had similar specific radioactivities after in vitro synthesis, but after in vivo radiolabeling the specific radioactivity of the terminal fructosyl moiety was significantly less than the internal fructosyl moiety. Evidence is presented that the uneven specific radioactivity of in vivo radiolabeling results from enzymatic transfer of terminal fructosyl residue from 1-kestose to sucrose.