Plant Physiology 97:217-226 (1991)
© 1991 American Society of Plant Biologists
Metabolism and Enzymology
Biochemical Genetics of Plant Secondary Metabolites in Arabidopsis thaliana 1
The Glucosinolates
George W. Haughn,
Laurence Davin2,
Michael Giblin and
Edward W. Underhill
Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada,
Plant Biotechnology Institute, Saskatoon, Saskatchewan S7N 0W9, Canada
Mutants of Arabidopsis thaliana with a glucosinolate content different from wild type were isolated by screening a mutagenized population of plants. Six mutants were detected out of a population of 1200 screened. One of these mutants, TU1, was analyzed in detail. Leaf and seed tissues of line TU1 lack or have reduced amounts of many of the aliphatic glucosinolates found in the wild type due to a recessive allele, gsm1, of a single nuclear gene, GSM1. The seed phenotype is inherited as a maternal effect suggesting that the embryo is dependent on the maternal tissue for its glucosinolates. Experiments involving feeding of 14C-labeled intermediates suggested that the gsm1 allele results in a metabolic block which decreases the availability of several amino acid substrates required for glucosinolate biosynthesis: 2-amino-6-methylthiohexanoic acid, 2-amino-7-methylthioheptanoic acid, and 2-amino-8-methylthiooctanoic acid. The mutation does not result in any obvious changes in morphology or growth rate. A pathway for the biosynthesis of glucosinolates in A. thaliana is proposed.
2 Present address: Institute of Biological Chemistry, Washington State University. 467 Clark Hall, Pullman, WA 99164.
1 This work was supported in part by a grant from the Natural Science and Engineering Research Council of Canada (No. OGP0036718 to G.W.H.), and two contracts with the National Research Council of Canada (No. 31964-8-0001/01-SS and 31964-7-0005/01-SG to G.W.H.) Issued as manuscript NRCC No. 32465.
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