Plant Physiology 97:55-59 (1991)
© 1991 American Society of Plant Biologists
Metabolism and Enzymology
Circadian Rhythm in Amino Acid Uptake by Synechococcus RF-1 1
Tsung-Hsien Chen,
Tzy-Li Chen,
Liang-Ming Hung and
Tan-Chi Huang
Institute of Botany, Academia Sinica, Taipei, Taiwan 11529, Republic of China
In the prokaryote Synechococcus RF-1, circadian changes in the uptake of L-leucine and 2-amino isobutyric acid were observed. Uptake rates in the light period were higher than in the dark period for cultures entrained by 12/12 hour light/dark cycles. The periodic changes in L-leucine uptake persisted for at least 72 hours into continuous light (L/L). The rhythm had a free-running period of about 24 hours in L/L at 29°C. A single dark treatment of 12 hours could initiate rhythmic leucine uptake in an L/L culture. The phase of rhythm could be shifted by a pulse of low temperature (0°C). The free-running periodicity was "temperature-compensated" from 21 to 37°C. A 24 hour depletion of extracellular Ca2+ before the free-running L/L condition reduced the variation in uptake rate but had little effect on the periodicity of the rhythm. The periodicity was also not affected by the introduction of 25 mM NaNO3. The uptake rates for 20 natural amino acids were studied at 12 hour intervals in cultures exposed to 12/12 hour light/dark cycles. For eight of these amino acids (L-Val, L-Leu, L-Ile, L-Pro, L-Phe, L-Trp, L-Met, and L-Tyr), the light/dark uptake rate ratios had values greater than 3 and the rhythm persisted in L/L.
1 Supported by grants from National Science Council (NSC79-0211-B001-27) and Academia Sinica. Republic of China.
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