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Microbe-Plant Interactions

A Lipoxygenase Pathway Is Activated in Rice after Infection with the Rice Blast Fungus Magnaporthe grisea

Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan, Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan

Lipoxygenase (LOX) and lipid hydroperoxide-decomposing activity (LHDA) markedly increased in the fifth leaves of rice (Oryza sativa cv Aichiasahi) after infection with the rice blast fungus, Magnaporthe grisea. The increases in the enzyme activities were significantly higher in response to infection with an incompatible strain (race 131) compared with infection with a compatible strain (race 007) of the fungus. Using ion-exchange chromatography, we isolated three LOX activities (leaf LOX-1, -2, -3) from both uninoculated and infected leaves. The activity of leaf LOX-3, in particular, increased in the incompatible race-infected leaves. The leaf LOX-3 had a pH optimum of 5.0 and produced preferentially 13-L-hydroperoxy-9,11 (Z,E)-octadecadienoic acid (13-HPODD) from linoleic acid. 13-HPODD and 13-L-hydroxy-9,11 (Z,E)-octadecadienoic acid, one of the reaction products from 13-HPODD by LHDA, were highly inhibitory to the germination of conidia of the fungus. The present study provides correlative evidence for important roles of LOX and LHDA in the resistance response of rice against the blast fungus.

² Present address: Yakult Central Institute for Microbiological Research, 1796 Yano Kunitachi-shi, Tokyo 186, Japan.

³ Present address: Mitsui Plant Biotechnology Research Institute, A-10 Tsukuba Center Inc., Sengen 2-1-6, Tsukuba, Ibaraki 305, Japan.

⁴ Present address: Fuji Oil Ctd., Tsukuba R&D Center, Tsukuba Ibaraki 324, Japan.

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