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Plant Physiology 97:562-568 (1991)
© 1991 American Society of Plant Biologists

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Environmental and Stress Physiology

NaCl Regulation of Tonoplast ATPase 70-Kilodalton Subunit mRNA in Tobacco Cells 1

Meena L. Narasimhan, Marla L. Binzel, Eva Perez-Prat, Zutang Chen, Donald E. Nelson, Narendra K. Singh, Ray A. Bressan and Paul M. Hasegawa

Center for Plant Environmental Stress Physiology, Department of Horticulture, Purdue University, West Lafayette, Indiana 47907, Texas A&M Research and Extension Center, El Paso, Texas 79927, Departmento Bioquimica Y Biologia Molecular, Facultad Ciencias, Universidad de Malaga, 29071 Malaga, Spain, Department of Botany and Microbiology, Auburn University, Auburn, Alabama 36849-5407

A cDNA clone encoding the 70-kilodalton subunit of the tobacco (Nicotiana tabacum var Wisconsin 38) tonoplast ATPase has been isolated. The 1.656 kilobase insert contains only open reading frame that represents more than 80% of the carrot cDNA coding region. The deduced amino acid sequence has greater than 95% sequence identity with the homologous carrot sequence. A transcript of approximately 2.7 kilobase was detected on Northern blots of tobacco poly(A)+ selected or total RNA using labeled probe produced from this clone. The gene was expressed throughout the growth cycle in unadapted and 428 millimolar NaCl adapted cells. Transcription of the 70-kilodalton subunit gene or mRNA stability was induced by short-term NaCl treatment in NaCl adapted cells or by abscisic acid treatment in both adapted and unadapted cells. Southern analysis indicated the presence of up to four genes encoding the 70-kilodalton subunit.

1 This research was supported by U.S. Department of Agriculture grant No. 85-CRCR-1-1670. Journal paper No. 13034 of the Purdue University Agricultural Experiment Station.




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