Plant Physiology 97:588-592 (1991)
© 1991 American Society of Plant Biologists
Environmental and Stress Physiology
Postanoxic Injury in Soybean (Glycine max) Seedlings 1
Tara T. VanToai and
Christopher S. Bolles
Soil Drainage Research Unit, Agricultural Research Service, United States Department of Agriculture, and Department of Agronomy, The Ohio State University, Columbus, Ohio 43210
The postanoxic injury, also known as reperfusion injury, is associated with the returning of anoxic tissues to normal atmosphere. Using tetrazolium chloride staining, ATP content, and seedling growth rate as indicators, we found that postanoxic injuries in soybean (Glycine max) seedlings were more severe after 1 and 2 hours of anoxia than after longer anoxic durations (3 to 5 hours). Anaerobic incubation of root tips in the presence of 100 mM ascorbate, an antioxidant and free radical-scavenging compound, alleviated the postanoxic injury associated with the short durations of anoxia. Extracts from soybean seedling roots returned to air from 1 hour of anoxia had an elevated capacity to produce superoxide radicals over extracts from postanoxic roots stressed for 3 or 5 hours. Activity of superoxide dismutase in soybean roots returned to air from 1 and 2 hours of anoxia was 30 to 50% lower than activities in roots returned to air from 5 hours of anoxia. Superoxide dismutase-specific transcripts were also lower in postanoxic roots stressed for 1 hour than in roots stressed for longer anoxic durations. The evidence suggested that the postanoxic injury of soybean roots after a short anoxic stress was associated with an increased superoxide radicals production capacity coupled with a reduced superoxide dismutase activity. Periods of anoxia of at least 3 hours were necessary for soybean seedlings to develop the ability to cope with postanoxic stress.
1 This work was supported in part by a Grant-in-Aid of Research to C.S.B. from Sigma Xi society. Contribution of Soil Drainage Research Unit, U.S. Department of Agriculture-Agricultural Research Station, and the Ohio Agricultural Research and Development Center, Wooster, OH. Ohio Agricultural Research and Development Center journal article No. 22-91.
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