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Development and Growth Regulation

Differential Effects of Elicitors on the Viability of Rice Suspension Cells ¹

Laboratorium voor Genetica, Universiteit Gent, B-9000 Gent, Belgium

We have compared the effects of two elicitors of defense-related processes on rice (Oryza sativa L.) suspension cells. Both chitosan and salicylic acid induced the accumulation of extracellular chitinase, thickening of the cell wall, and a variety of cytological changes in treated cells. Chitosan also induced the production of a brown pigment and cell death. Both of these effects depended on the availability of reactive oxygen species, because the damage was greatly reduced by either catalase or free-radical scavengers. Pretreating cells with salicylic acid also protected them from the cytotoxic effects of chitosan. This type of induced tolerance persisted when salicylic acid was removed and was not simply due to the release of extracellular substances, because salicylic acid-treated cells did not protect untreated cells from chitosan-induced death. Salicylic acid also stimulated the production of a 10-kilodalton subtilisin inhibitor that was not produced by chitosan-treated cells. Most of these changes are associated with the hypersensitive response of many plant species, including monocotyledons, and may serve as an in vitro model for investigating the biochemistry of some diseases.

¹ This work was supported by grants from the Services of the Prime Minister (U.I.A.P. 12OC0187), the "Vlaams Actieprogramma Biotechnologie" (174KP490), and the Rockefeller Foundation (RF 89025 No. 95). C. Masuta was supported by a grant from Tobacco Inc.