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Environmental and Stress Physiology

Effect of Sucrose on Phase Behavior of Membranes in Intact Pollen of Typha latifolia L., as Measured with Fourier Transform Infrared Spectroscopy ¹

Department of Plant Physiology, Agricultural University, Wageningen, The Netherlands, Department of Zoology, University of California, Davis, California 95616

In previous studies, we have shown that the temperature dependent vibrational frequency of the CH₂ stretch in hydrocarbons in intact pollen grains can be recorded with Fourier transform infrared spectroscopy and used to measure phase transition temperatures (T_m) in these hydrocarbons. Circumstantial evidence was provided that the major contribution to the signal seen in these samples was from membrane phospholipids, and that sucrose in the dry pollen grains reduced T_m of those phospholipids. In the current study, we clarify why a major constituent of the pollen grains, neutral lipids contained in discrete lipid droplets, does not contribute significantly to the signal. Further, we have isolated membranes from the pollen and show that T_m in the isolated membranes rises from –6°C in the hydrated membranes to 58°C when the membranes are dried without the addition of sucrose. However, when the isolated membranes are dried in the presence of increasing amounts of sucrose, T_m fell steadily, reaching a minimal value of 31°C, a figure in good agreement with that seen in the intact pollen grains. The amount of sucrose required to depress T_m maximally in these membranes is also apparently in agreement with that found in the intact pollen, suggesting that sucrose depresses T_m in the pollen.

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