Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 97:863-872 (1991)
© 1991 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (111)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Foyer, C.
Right arrow Articles by Kunert, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Foyer, C.
Right arrow Articles by Kunert, K. J.
Agricola
Right arrow Articles by Foyer, C.
Right arrow Articles by Kunert, K. J.
Environmental and Stress Physiology

Effects of Elevated Cytosolic Glutathione Reductase Activity on the Cellular Glutathione Pool and Photosynthesis in Leaves under Normal and Stress Conditions

Christine Foyer, Maud Lelandais, Camille Galap and Karl Josef Kunert1

Laboratoire du Métabolisme, Institut National de la Recherche Agronomique Route de St-Cyr, 78026 Versailles cedex, France, Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique Route de St-Cyr, 78026 Versailles cedex, France

Tobacco (Nicotiana tabacum var Samsun) was transformed using the bacterial gor gene coding for the enzyme glutathione reductase. Transgenic plants were selected by their kanamycin resistence and expression of the bacterial gor gene. After separation by isoelectric focusing techniques, leaf extracts from transgenic plants having both native and bacterial glutathione reductase activity gave, in addition to the six bands of the native enzyme, two further closely running isoenzymes. These additional bands originating from the expression of the bacterial gor gene were nonchloroplastic. Leaves from transgenic plants had two- to 10-fold higher glutathione reductase activity than non-transgenic controls. The amount of extractable glutathione reductase activity obtained in transgenic plants was dependent on leaf age and the conditions to which leaves were exposed. Both light and exposure to methylviologen increased leaf glutathione reductase activity. Elevated levels of cytosolic glutathione reductase activity in transgenic plants had no effect on the amount or reduction state of the reduced glutathione/oxidized glutathione pool under optimal conditions or oxidative conditions induced by methylviologen. The glutathione pool was unaltered despite the oxidation-dependent loss of CO2 assimilation and oxidation of enzymes involved in photosynthesis. However, the reduction state of the ascorbate pool was greater in transgenic plants relative to nontransgenic controls following illumination of methylviologen-treated leaf discs. Therefore, we conclude that in the natural state glutathione reductase is present in tobacco at levels above those required for maximal operation of the ascorbate-glutathione pathway.

1 Present address: AECI Limited, Research and Development Department, PO Modderfontein 1645, South Africa.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
E. Valero, M. I. Gonzalez-Sanchez, H. Macia, and F. Garcia-Carmona
Computer Simulation of the Dynamic Behavior of the Glutathione-Ascorbate Redox Cycle in Chloroplasts
Plant Physiology, April 1, 2009; 149(4): 1958 - 1969.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Henmi, T. Demura, S. Tsuboi, H. Fukuda, M. Iwabuchi, and K. Ogawa
Change in the Redox State of Glutathione Regulates Differentiation of Tracheary Elements in Zinnia Cells and Arabidopsis Roots
Plant Cell Physiol., November 1, 2005; 46(11): 1757 - 1765.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Potters, N. Horemans, S. Bellone, R. J. Caubergs, P. Trost, Y. Guisez, and H. Asard
Dehydroascorbate Influences the Plant Cell Cycle through a Glutathione-Independent Reduction Mechanism
Plant Physiology, April 1, 2004; 134(4): 1479 - 1487.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
Y. I. Kuk, J. S. Shin, N. R. Burgos, T. E. Hwang, O. Han, B. H. Cho, S. Jung, and J. O. Guh
Antioxidative Enzymes Offer Protection from Chilling Damage in Rice Plants
Crop Sci., November 1, 2003; 43(6): 2109 - 2117.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Zaka, C. M. Vandecasteele, and M. T. Misset
Effects of low chronic doses of ionizing radiation on antioxidant enzymes and G6PDH activities in Stipa capillata (Poaceae)
J. Exp. Bot., September 1, 2002; 53(376): 1979 - 1987.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Noctor, L. Gomez, H. Vanacker, and C. H. Foyer
Interactions between biosynthesis, compartmentation and transport in the control of glutathione homeostasis and signalling
J. Exp. Bot., May 15, 2002; 53(372): 1283 - 1304.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Tommasi, C. Paciolla, M. C. de Pinto, and L. D. Gara
A comparative study of glutathione and ascorbate metabolism during germination of Pinus pinea L. seeds
J. Exp. Bot., August 1, 2001; 52(361): 1647 - 1654.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. Dixit, V. Pandey, and R. Shyam
Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad)
J. Exp. Bot., May 1, 2001; 52(358): 1101 - 1109.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1991 by the American Society of Plant Biologists