Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 98:157-162 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Differential Protein Accumulation in Banana Fruit during Ripening 1

Eva Dominguez-Puigjaner, Miguel Vendrell and M. Dolors Ludevid

Departamento de Biologia Molecular y Agrobiologia, Centro de Investigación y Desarrollo, Consejo Superior de Investigaciones Científicas, Jordi Girona Salgado 18-26, 08034 Barcelona, Spain, Departamento de Genética Molecular, Centro de Investigación y Desarrollo, Consejo Superior de Investigaciones Científicas, Jordi Girona Salgado 18-26, 08034 Barcelona, Spain

Banana (Musa acuminata, cv Dwarf Cavendish) proteins were extracted from pulp tissue at different stages of ripening and analyzed by two-dimensional electrophoresis. The results provide evidence of differential protein accumulation during ripening. Two sets of polypeptides have been detected that increase substantially in ripe fruit. These polypeptides were characterized as glycoproteins by western blotting and concanavalin A binding assays. Antibodies againts tomato polygalacturonase cross-react with one of these sets of proteins.


1 This work was supported by grant ALI-88-0138 from Comisión Interministerial de Ciencia y Technologia, and grant AR/88 from the Comissió Interdepartamental de Recerca e Innovació Tocnologica Generalitat de Catalunya. E.D.-P. is a recipient of a fellowship from the Ministerio Educatión y Ciencia.







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