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Plant Physiology 98:387-391 (1992)
© 1992 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

5'CATGCAT-3' Elements Modulate the Expression of Glycinin Genes

Jean-Marc Lelievre1, Luiz O. Oliveira and Niels C. Nielsen

United States Department of Agriculture, Agricultural Research Service, Purdue University, West Lafayette, Indiana 47907, Department of Agronomy, Purdue University, West Lafayette, Indiana 47907

Promoters of most seed proteins in legumes contain one or more 5'-CATGCAT-3' elements. To test if these elements have a function in the expression of these genes, the 2.3 kilobase pairs Gy2 glycinin promoter was ligated to a {beta}-glucuronidase reporter sequence and transformed into tobacco. Elimination of a 5'-CATGCAT-3' element 101 base pairs upstream from the transcription start site in the construction caused about a 10-fold reduction in the amount of {beta}-glucuronidase activity compared with when the element was present in the gene. Elimination of 1.9 kilobase pairs from the 5'-end of the promoter caused a two-to threefold reduction in activity. The results show that the 5'-CATGCAT-3' element plays a role in regulating the amount of expression from the gene, but that there are also other factors farther upstream from the gene that affect the level of expression.

1 Current address: ENSAT, Institute National Polytechnique de Toulouse, 145 Avenue de Muret, 31076 Toulouse, France.




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Copyright © 1992 by the American Society of Plant Biologists