This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Coleman, G. D. Articles by Fuchigami, L. H. Search for Related Content PubMed PubMed Citation Articles by Coleman, G. D. Articles by Fuchigami, L. H. Agricola Articles by Coleman, G. D. Articles by Fuchigami, L. H.

Molecular Biology and Gene Regulation

Complementary DNA Cloning of Poplar Bark Storage Protein and Control of Its Expression by Photoperiod ¹

Department of Horticulture, Oregon State University, Corvallis, Oregon 97331, Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon 97331

Bark storage proteins accumulate in the bark of many woody plants during autumn and winter. In poplar (Populus deltoides Bartr. ex Marsh), the accumulation of the 32-kilodalton bark storage protein is controlled by photoperiod. We have isolated a full-length cDNA encoding for the poplar 32-kilodalton bark storage protein and determined its nucleotide sequence. The derived amino acid sequence shows that poplar bark storage protein is rich in serine, leucine, phenylalanine, and lysine. Poplar bark storage protein is similar to the poplar wound-induced cDNA clone 4 and clone 16 (TJ Parsons, HD Bradshaw, MP Gordon [1989] Proc Natl Acad Sci USA 86: 7895-7899). DNA gel blot analysis suggests that poplar bark storage protein is encoded by a multigene family of about five genes. Poplar plants grown in long days contained low levels of mRNA for the bark storage protein. Exposure to short days resulted in an increase in bark storage protein mRNA within 7 days. After 21 days of short day exposure, high levels of mRNA were detected. The accumulation of bark storage protein mRNA in response to short days was also observed in plants exposed to natural shortening daylengths. Our results indicate that the accumulation of poplar bark storage protein mRNA is controlled by photoperiod. This finding will provide a useful system for investigating photoperiodism in woody plants.

This article has been cited by other articles:

				C. Dhont, Y. Castonguay, J.-C. Avice, and F.-P. Chalifour VSP accumulation and cold-inducible gene expression during autumn hardening and overwintering of alfalfa J. Exp. Bot., July 1, 2006; 57(10): 2325 - 2337. [Abstract] [Full Text] [PDF]

				L. Rossato, P. Laine, and A. Ourry Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns J. Exp. Bot., August 1, 2001; 52(361): 1655 - 1663. [Abstract] [Full Text] [PDF]