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Plant Physiology 98:749-752 (1992)
© 1992 American Society of Plant Biologists

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Environmental and Stress Physiology

Low Measuring Temperature Induced Artifactual Increase in Chlorophyll a Fluorescence 1

Norman P. A. Huner2, Gunnar Öquist and Lars-Göran Sundblad

Department of Plant Physiology, University of Umeå, Umeå, Sweden, Institutet för Skogsförbättring, Norra Distriktet, Sävar, Sweden

Measurement of in vivo chlorophyll a fluorescence at temperatures lower than 20°C can cause an artifactual, nonphotochemically related overestimation of variable fluorescence leading to the calculation of negative values for the nonphotochemical quenching parameter and an underestimation of the photochemical quenching parameter. This artifact is observed only upon exposure of the leaf sample to actinic light. We suggest that a temperature differential between the fiber-optic probe and the leaf sample results in the deposition of water vapor on the probe that distorts the light path such that an increased modulated fluorescence signal is observed. This artifact is eradicated by ensuring that the end of the fiber-optic probe is kept free of condensation.

2 Permanent address: Department of Plant Science, University of Western Ontario, London, Ontario, Canada N6A 5B7.

1 This research was supported by a Natural Sciences and Engineering Research Council of Canada Operational Grant and an Organization for Economic Cooperation and Development (Project on Food Production and Preservation) Visiting Research Fellowship to N.P.A.H. and a grant from the Swedish Natural Science Research Council to G.O.






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Copyright © 1992 by the American Society of Plant Biologists