Plant Physiol. Drug Metab Dispos
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Plant Physiology 98:1029-1034 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Some Properties of a Functional Reconstituted Plasmalemma H+-ATPase Activated by Fusicoccin 1

Mauro Marra, Alessandro Ballio, Maria Rosaria Fullone and Patrizia Aducci

Dipartimento di Biologia, II Università di Roma "Tor Vergata," V. O. Raimondo, 00173 Rome, Italy, Dipartimento di Scienze Biochimiche "A. Rossi Fanelli," Università di Roma "La Sapienza," P.le Aldo Moro 5, 00185 Rome, Italy

Fusicoccin was shown to stimulate the ATP-driven, intravesicular acidification of liposomes reconstituted with crude fusicoccin receptors and the H+-translocating ATPase, both solubilized from maize (Zea mays L.) plasma membrane. The present paper reports optimal conditions for dual reconstitution and fusicoccin activation as well as the biochemical characterization of the effect of fusicoccin on this system. Fusicoccin stimulation of proton pumping was dependent on pH and fusicoccin concentration. Its specificity was demonstrated by the positive effect of two cotylenins that have a high affinity for fusicoccin receptors and by the negative response to 7,9-epideacetylfusicoccin, an inactive fusicoccin derivative. Kinetic measurements at different ATP concentrations showed that fusicoccin increases the Vmax of the enzyme. Fusicoccin stimulation of maize H+-ATPase was also maintained when receptors from maize were substituted by those from spinach (Spinacia oleracea L.).

1 The research has been supported by the National Research Council of Italy, Special Project RAISA, Sub-project No. 2 Paper No. 268. The work has also been partially supported by the Italian Ministry of Agriculture and Forestry and by a contract of the Commission of European Communities (BRIDGE). A.B. acknowledges the financial support of the National Research Council special ad hoc program "Chimica Fine 2," subproject 3, and of the Italian Ministry of University and Scientific Research.




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L. Camoni, V. Iori, M. Marra, and P. Aducci
Phosphorylation-dependent Interaction between Plant Plasma Membrane H+-ATPase and 14-3-3 Proteins
J. Biol. Chem., March 31, 2000; 275(14): 9919 - 9923.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Society of Plant Biologists