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Metabolism and Enzymology

Immunological Detection of Acetaldehyde-Protein Adducts in Ethanol-Treated Carrot Cells ¹

Dipartimento di Biologia delle Piante Agrarie, Universita' di Pisa, viale delle Piagge 23, I-56124 Pisa, Italy, Centro Ricerche Sclavo, via Fiorentina 1, I-53100 Siena, Italy

Polyclonal antibodies able to recognize protein-acetaldehyde conjugates were produced and characterized. The antibodies react with sodium cyanoborohydride-reduced Schiff's bases between acetaldehyde and a protein, independently of the nature of the macromolecule binding the acetaldehyde moiety. Only conjugates between acetaldehyde or propionaldehyde and a protein are recognized; conjugates obtained with other aldehydes are not reactive. Results concerning the formation of acetaldehyde adducts with carrot (Daucus carota L.) proteins are presented as well as the presence of such conjugates in ethanol-treated carrot cell cultures, a system highly sensitive to the presence of ethanol in the culture medium.

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