Plant Physiology 98:927-935 (1992)
© 1992 American Society of Plant Biologists
Metabolism and Enzymology
Subcellular Distribution of O-Acetylserine(thiol)lyase in Cauliflower (Brassica oleracea L.) Inflorescence
Norbert Rolland,
Michel Droux and
Roland Douce
Unité Mixte Centre National de la Recherche Scientifique/Rhône-Poulenc (Unité Associée au Centre National de la Recherche Scientifique, U.M. 41), Rhône-Poulenc Agrochimie, 14-20 impasse Pierre Baizet, 69263 Lyon Cedex 09, France
The subcellular localization of O-acetyiserine(thiol)lyase (EC 4.2.99.8) in nongreen tissue from higher plants has been studied using purified proplastids, mitochondria, and protoplasts from cauliflower (Brassica oleracea L.) buds as a source of subcellular fractions. O-Acetylserine(thiol)lyase has been detected in both organelles (proplastids and mitochondria) and a cytosolic extract obtained by protoplast fractionation. We confirmed these observations, demonstrating that a form of the enzyme different in global charge and separated from others by anion-exchange chromatography corresponded to each subcellular location. Our observations are consistent with the need for cysteine biosynthesis in each subcellular compartment where the synthesis of proteins occurs.
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