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Plant Physiology 98:962-965 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Nondwarf Rice Seedling Bioassay for Gibberellins 1

Takaaki Nishijima, Masaji Koshioka and Hiroko Yamaji

National Research Institute of Vegetables, Ornamental Plants and Tea, Ministry of Agriculture, Forestry and Fishery of Japan, Kusawa, Ano-cho, Mie, 514-23 Japan

The use of nondwarf rice (Oryza sativa L.) cultivars treated with uniconazole as test plants for gibberellin (GA) bioassay instead of Tan-ginbozu dwarf rice variant was investigated. The sensitivity of six nondwarf rice cultivars to GAs was increased substantially by treatment of the seeds with uniconazole. The minimum detectable dose of a GA in the nondwarf cultivars treated with uniconazole was 1- to 1/10-fold of that in the nontreated Tanginbozu and 3- to 10-fold of that in uniconazole-treated Tanginbozu. The relative activity of several GAs on treated nondwarf rice cultivars was not largely different from that to Tan-ginbozu. Considering that seeds of nondwarf rice are available commercially, the nondwarf rice seedling assay would be useful as a simple assay for systematic analysis of GAs, and also as a routine teaching tool in high schools and universities.

1 This study was performed through Special Coordination Funds of the Science and Technology Agency of the Japanese Government.




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A. Ikeda, M. Ueguchi-Tanaka, Y. Sonoda, H. Kitano, M. Koshioka, Y. Futsuhara, M. Matsuoka, and J. Yamaguchi
slender Rice, a Constitutive Gibberellin Response Mutant, Is Caused by a Null Mutation of the SLR1 Gene, an Ortholog of the Height-Regulating Gene GAI/RGA/RHT/D8
PLANT CELL, May 1, 2001; 13(5): 999 - 1010.
[Abstract] [Full Text]


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Copyright © 1992 by the American Society of Plant Biologists